酪蛋白胶束粉的陶瓷膜分离生产工艺  

Pilot scale production process of micellar casein concentrate powder

作　　者：陈建行[1] 刘鹭[1] 孙颜君[1,2] 苏燕玲[1] 张书文[1] 孔凡丕[1] 蒋士龙 吕加平[1] 

机构地区：[1]中国农业科学院农产品加工研究所,农业部农产品加工综合性重点实验室,北京100193 [2]西北农林科技大学食品科学与工程学院,杨凌712100 [3]黑龙江飞鹤乳业有限公司,齐齐哈尔161800

出　　处：《农业工程学报》2013年第9期256-266,共11页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家十二五科技支撑课题资助(2011BAD09B01);北京市重大科技计划项目资助(D101105046010002)

摘　　要：为尽快建立酪蛋白胶束粉(micellar casein concentrate,MCC)的中试生产线,实现MCC在国内的工业化生产,该研究通过测定牛乳蛋白粒径,选取孔径40和100nm的中空纤维陶瓷膜进行膜分离效果的对比,并选用膜分离效果较佳的陶瓷膜进行最佳操作参数的确定及稀释过滤工艺的研究,最后将自制MCC和进口MCC进行品质特性对比并分析其工业化生产的可能性。结果表明:孔径40nm中空纤维陶瓷膜与孔径100nm中空纤维陶瓷膜相比,渗透液中不含酪蛋白,能够减少酪蛋白损失,更适合于酪蛋白和乳清蛋白的分离,孔径40nm中空纤维陶瓷膜的最佳操作参数为操作压力2×105Pa,料液温度50℃;采用四段式的连续稀释过滤工艺,可使料液中酪蛋白占真蛋白的比例从69.39%提升至93.34%,真蛋白占干物质的比例从38.45%提升至88.15%;稀释过滤完毕后膜的纯水通量衰减了39.98%,经生物酶清洗液清洗后膜的纯水通量恢复至初始的98.02%;自制MCC在成分组成上与加拿大Proteinco公司生产的MCC相比差异不显著(p<0.05),但在溶解度和粉粒的微观形态上优于ProteincoMCC;生产1kgMCC需要原料乳46.24L,成本为244.58元,可实现其国内工业化生产。研究结果为实现MCC在国内工业化生产及其应用性研究提供参考。Micellar casein concentrate （MCC） powder, a new milk protein product produced by a combination of processes including membrane separation, evaporation and spray drying has potential applications in cheese making, whipped topping, coffee whitener and yogurt making. It is becoming more and more popular in the food processing industry, but currently China depends on imported MCC mainly from developed countries. The main objective of this study was to successfully develop a pilot scale production process for micellar casein concentrate powder and facilitate its rapid adoption and manufacturing in China’s dairy plants. Preliminary studies involved the selection of a suitable hollow fiber ceramic membrane for the separation of casein micellar and serum protein between 100 nm and 40 nm pore size hollow fiber ceramic membranes （InoCep, Hyflux Ltd, Singapore）. The 40nm pore size hollow fiber ceramic membrane, which had better separation performance, was chosen for further investigation on optimum operating parameters, mainly temperature and pressure. A four-stage diafiltration process was employed, in which chemical composition and flux changes were measured at the end of every stage in the production line. Finally, the micellar casein concentrate powder made after evaporation and spray drying process was compared with commercial MCC （Proteinco Inc., Quebec, Canada） in terms of their physical characteristics and chemical compositions. The yield rate and the production costs were also calculated. The results showed that： （1） the permeate of 100 nm pore size membrane was cloudy but the permeate of 40 nm pore size membrane was clear in appearance and the proportion of casein in the true protein of the permeate for the 100 and 40 nm pore size membrane were 40.03% and 17.48%, respectively. Therefore, the 40nm pore size membrane was found to be more suitable for the separation of casein micellar and serum protein and its average permeate flux during a 3×concentration at the optimal operating conditions （t

关 键 词：膜 分离 过滤 酪蛋白胶束粉 陶瓷膜 

分 类 号：TS252.41[轻工技术与工程—农产品加工及贮藏工程]