大孔树脂对罗非鱼皮胶原蛋白抗氧化肽脱盐作用的研究  

Desalination of Macroporous Absorption Resin on Antioxidant Peptides from Tilapia Skin Collagen

作　　者：夏光华[1] 申铉日[1] 酒志强[1] 黄霜霞[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《现代食品科技》2013年第5期1052-1056,共5页Modern Food Science and Technology

基　　金："十二五"农村领域国家科技计划课题(2012BAD28B06);海口市重点科技计划项目(2011-079)

摘　　要：为了探索罗非鱼皮胶原蛋白抗氧化肽的最佳脱盐工艺,本文采用DA201-C大孔吸附树脂对抗氧化肽进行脱盐试验。结果表明:DA201-C大孔吸附树脂对抗氧化肽的静态吸附率和吸附量分别为82.56%和54 mg/g;动态吸附和解吸最佳的上样浓度为60mg/mL,最佳上样流速为1 mL/min,解吸剂为75%乙醇。抗氧化肽经过DA201-C大孔吸附树脂处理后脱盐率为97.13%,回收率为81.16%,质量浓度为0.245 mg/mL时对ABTS自由基的清除率提高至39.58%。采用DA201-C大孔吸附树脂对罗非鱼皮胶原蛋白抗氧化肽进行脱盐处理是一种简便有效的方法。The desalting technology of antioxidant peptides fxom tilapia skin collagen by DA201-C macroporous adsorption resin was investigated. The results showed that the static absorption rate and absorbing capacity were 82.56% and 54mg/g, respectively. The best loading sample concentration, sampling flow rate and eluent were 60 mg/mL, 1 mL/min and 75% alcohol, respectively. Under these conditions, the desalination rate was 97.13% and recovery of peptides was 81.16%. The antioxidant activity was increased to 39.58% at the ~ncentration of 0.245 mg/mL. In conclusion, it was available and effective to desalt the antioxidant peptides fx0m tilapia skin collagen by using DA201-C maeroporous absorption resin.

关 键 词：罗非鱼 抗氧化肽 脱盐 大孔杠悃旨 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]