ROCK抑制剂Y-27632对Hep-2细胞中RhoC及ROCK表达的影响  

Effect of ROCK Inhibitor Y-27632 on RhoC and ROCK Expression in Hep-2 Cells

作　　者：贺雪[1] 刘良发[1] 郭维维[1] 

机构地区：[1]解放军总医院耳鼻咽喉头颈外科,解放军耳鼻咽喉研究所,北京100853

出　　处：《医学研究杂志》2013年第5期78-81,共4页Journal of Medical Research

基　　金：国家自然科学基金面上项目(30872863);军队"十一五"基金资助项目(06H042)

摘　　要：目的探讨ROCK抑制剂Y-27632对喉癌细胞系Hep-2中RhoC及ROCK表达的影响。方法在体外培养的喉癌细胞系Hep-2的培养液中加入ROCK抑制剂Y-27632后,用免疫荧光法,RT-PCR及Western blot检测喉癌细胞Hep-2细胞系RhoC及ROCK-1,ROCK-2的mRNA及蛋白的表达水平。结果经过Y-27632处理后,Hep-2中RhoC及ROCK-1和ROCK-2在细胞的mRNA及蛋白的表达下降。结论 Y-27632可抑制ROCK-1、ROCK-2及RhoC的表达。Objective To investigate the expression of RhoC and ROCK on the laryngeal carcinoma cell in vitro by treating Hep -2 cells with ROCK inhibitor Y - 27632. Methods The expression of RhoC, ROCK - 1 and ROCK - 2 in Hep - 2 cells was determined by RTPCR and Western blot assay when ROCK was inhibited before and after. Results After treatment with Y -27632, Hep -2 RhoC and ROCK1 and ROCK2 in the cells of mRNA and protein expression decreased. Conclusion Y - 27632 can effectively inhibit the expression of RhoC, ROCK - 1 and ROCK - 2 protein and mRNA in Hep - 2 cells.

关 键 词：RHOC ROCK Y-27632 HEP-2细胞 

分 类 号：R739.650.2[医药卫生—肿瘤]