蓝莓花色苷对大鼠视网膜光损伤的保护作用及其机制  

Protective Effect and Mechanism of Blueberry Anthocyanins against Retinal Photodamage in Rats

作　　者：孟宪军[1] 毕菀芩[1] 张琦[1] 李斌[1] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866

出　　处：《食品科学》2013年第11期242-245,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD31B05-3);辽宁省科技厅科技攻关项目(2011205001)

摘　　要：选取50只SD大鼠随机分为正常对照组、光损伤模型组及蓝莓花色苷(BA)低、中、高剂量组。观察大鼠视网膜形态学变化、测量视网膜外核层厚度、测定视网膜总蛋白含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和乳酸脱氢酶(LDH)活性,评价BA对大鼠视网膜光损伤(RP)保护作用,并探讨作用机理。结果表明:BA中、高剂量组与光损伤模型组比较,视网膜结构层次分明,细胞排列整齐;BA各剂量组可有效防止视网膜外核层厚度变薄及视网膜总蛋白含量的减少;BA中、高剂量组可显著提高视细胞SOD、GSH-Px的活性并降低MDA含量;BA各剂量组LDH活性较模型组无明显差异。蓝莓花色苷对大鼠视网膜光损伤有明显的保护作用,其机制可能与抗脂质过氧化作用有关。The aim of this study was to investigate the protective effect of blueberry anthocyanins （BA） against retinal photodamage in rats and elucidate the potenital mechanism. A total of 50 Sprague-Dawley rats were randomly divided into 5 groups： normal control group, model control group, low-dose group, medium-dose group, and high-dose group. The thickness of the retinal outer nuclear layer was measured as well as total retinal protein content and malondialhyde （MDA）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and lactate dehydrogenase （LDH） levels in the established photo-oxidative damage model. Compared with the model control group, rats administered with BA at medium dose and high dose had more clearly demarcated retinal structure and more ordered cells. Each dose of BA effectively prevented the thinning of the retinal outer nuclear layer and the reduction of total protein content. High-dose BA group also could significantly improve SOD, GSH-Px and MDA levels. Compared with the model control group, there was no signinificant difference in LDH level at any of the doses tested. Blueberry anthocyanins have a significant protective effect against rat retinal light damage, possibly because of their anti-lipid peroxidation activity.

关 键 词：蓝莓花色苷 光损伤 超氧化物歧化酶 乳酸脱氢酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]