几种常用的核染色标记物pH 6.0 Citrate和pH 9.0 Tris/EDTA修复液的比较  

Effect of pH 6.0 citrate buffer and pH 9.0 Tris/EDTA buffer on the common nuclear staining antibodies in antigen retrieval immunohisto chemistry

作　　者：张燕萍[1] 郭志琴[1] 唐正英[1] 王振[1] 张怡[1] 邬万新[1] 

机构地区：[1]浙江省嘉兴市第一医院,嘉兴314000

出　　处：《浙江创伤外科》2013年第3期315-317,共3页Zhejiang Journal of Traumatic Surgery

摘　　要：目的探讨pH 6.0 Citrate和pH 9.0 Tris/EDTA两种修复液在常用的免疫组化核染色标记物中的染色效果。方法选取本科室常用的3种核/浆标记抗体以及22种核标记抗体分别采用pH 6.0 Citrate和pH 9.0 Tris/EDTA微波热修复进行比较。结果在25种抗体中,其中有11种分别用pH 6.0 Citrate和pH 9.0 Tris/EDTA微波修复染色效果基本一致,在其余14种抗体中,有11种用pH 9.0 Tris/EDTA微波热修复方法进行修复时,阳性信号明显增强;而有3种抗体用pH 6.0 Citrate微波热修复方法进行修复时,阳性信号稍增强。结论对于这25种抗体来说,如果用微波加热的方法进行抗原修复,那么pH 9.0 Tris/EDTA优于PH 6.0 Citrate。Objective To study the staining effect of pH 6.0 citrate buffer and pH 9.0 Tris/EDTA buffer on the common nuclear staining anti- bodies in antigen retrieval immunohistochemistry. Methods 3 antibodies for both nuclear and cytoplasm staining and 22 antibodies for nuclear staining only were selected and compared in the microwave-based antigen retrieval immunohistochemistry using pH 6.0 citrate buffer and pH 9.0 Tris/ EDTA buffer, respectively. Results 11 of 25 antibodies using pH 9.0 Tris/EDTA buffer had higher the positive cell numbers than those using pH 6.0 citrate buffer. 3 of 25 antibodies using pH 9.0 Tris/EDTA buffer had fewer the positive cell numbers than those using pH 6.0 citrate buffer. The positive cell numbers in the 1 lof 25 antibodies using pH 9.0 Tris/EDTA buffer were similar to those using pH 6.0 citrate buffer. Conclusion The pH 9.0 Tris/EDTA buffer is superior to the PH 6.0 citrate buffer for the common nuclear staining antibodies in the microwave-based antigen retrieval immunohistochemistrv.

关 键 词：抗原修复技术 PH 6 0 CITRATE PH 9 0 TRIS EDTA 核染色标记物 

分 类 号：R783.3[医药卫生—口腔医学]