龙须菜中硫琼脂的体外抗氧化评价及降血糖、降血脂活性的动物试验  

Study on antioxidation,animal experiments of hyperglycemia and hyperlipemia of agaropectin from Gracilaria lemaneiformis

作　　者：刘秋凤[1,2] 吴成业[2] 苏永昌[2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建省水产研究所,福建厦门361013

出　　处：《南方水产科学》2013年第3期57-66,共10页South China Fisheries Science

基　　金：国家海洋公益性行业科研专项(201205022-4)

摘　　要：为探讨硫琼脂降血糖、降血脂活性及其体外抗氧化作用,文章通过对超氧阴离子(.O2-)、羟基自由基(.OH)及11-二苯基-2-三硝基苯肼(DPPH)清除效果的研究评价了硫琼脂的体外抗氧化活性;建立四氧嘧啶高血糖小鼠模型和高脂饲料高血脂大鼠模型,给予不同剂量的硫琼脂设计动物试验,以测定的小鼠空腹血糖值、大鼠血脂水平[胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]以及血清、肝脏的生化指标值研究硫琼脂的降血糖、降血脂活性。结果表明,硫琼脂具有良好的抗氧化作用,尤其对.OH的清除效果最好;能显著降低高血糖小鼠的空腹血糖值及高血脂大鼠的TC、TG、LDL-C浓度,提高HDL-C浓度,具有降血糖、降血脂活性。This paper aims at investigating the role agaropectin plays in hyperglycemia, hyperlipemia and antioxidation. We studied the cleaning effect of ？O2-, ？OH and DPPH, based on which vitro antioxidation was assessed. We also researched hyperglycemia and hyperlipidemia by making use of alloxan to build diabetic model, and hyperlipidemia fodder to build hyperlipemia model, and conducted animal experiments with different doses of agaropectin so as to assay fasting blood glucose, rat’s blood fats （TC, TG, HDL-C and LDL-C）, as well as biochemical indexes of serums and livers. The study of vitro antioxidation reveals that agaropectin has antioxidant effect, functioning especially well on scavenging ？OH. Animal experiments show that agaropectin can significantly reduce fasting plasma glucose value in hyperglycemia mice, and decrease the content of TC, TG and LDL-C contained in hyperlipidemia rats, which indicates that agaropectin really has an effect on hyperglycemia and hyperlipemia.

关 键 词：硫琼脂 抗氧化活性 高血糖症 高血脂症 

分 类 号：R285.5[医药卫生—中药学]