PTEN、nm23-H1和Syk在大肠癌中的表达及意义  

Expressions and significances of PTEN,nm23-H1 and Syk in colorectal cancer

作　　者：董华承[1] 范丽昕[1] 朴正爱[2] 王晓鸿[1] 王海[2] 王丽双[1] 

机构地区：[1]大连市第三人民医院肿瘤内科,辽宁大连116033 [2]大连市第三人民医院病理科,116033

出　　处：《临床肿瘤学杂志》2013年第5期403-407,共5页Chinese Clinical Oncology

基　　金：2011年大连市卫生局课题资助项目(2011-25)

摘　　要：目的检测PTEN、nm23-H1和Syk蛋白在大肠癌中的表达,探讨其与大肠癌发生、发展、浸润和转移的关系。方法采用免疫组化SP法检测60例大肠癌组织及30例正常大肠黏膜中PTEN、nm23-H1和Syk的表达,分析三者与大肠癌临床病理特征的关系。结果正常大肠黏膜中PTEN、nm23-H1和Syk的阳性表达率分别为90.0%(27/30)、93.3%(28/30)和96.7%(29/30),而在大肠癌组织中分别为36.7%(22/60)、41.7%(25/60)和31.7%(19/60),差异均有统计学意义(P<0.01)。大肠癌中3种蛋白的表达与年龄、性别、肿瘤大小和发生部位均无关(P>0.05),PTEN、Syk表达与大肠癌的浸润程度、淋巴结转移、分化程度及Duke's分期有关,nm23-H1表达与浸润程度、淋巴结转移及Duke's分期有关。大肠癌中3种蛋白表达两两呈正相关。结论 PTEN、nm23-H1和Syk表达与大肠癌发生、发展、浸润及转移密切相关,联合检测是判断大肠癌生物学行为的重要指标。Objective To evaluate the expressions of PTEN, nm23-H1 and Syk in colorectal cancer and their relationships with the carcinogenesis, development, invasion and metastasis of colorectal cancer. Methods The expression of PTEN, nm23-H1 and Syk proteins in 60 cancer tissues and 30 normal colorectal mucous tissues were determined by the immunohistochemieal SP method. Their correlations with clinicopathologic features were analyzed. Results The positive rates of PTEN, nm23-H1 and Syk expression was 90. 0% （27/30） ,93.3% （28/30） and 96. 7% （29/30） in normal tissues, higher than 36. 7% （22/60） ,41.7% （25/60） and 31.7% （19/60） in colorectal cancer tissues, respectively（ P 〈 0. 01 ）. The expression of the three proteins in coloreetal cancer tissues were not associated with age, gender, tumor size or tumor location（ P 〉 0.05 ）. The expressions of PTEN and Syk were significantly re- lated with differentiation, depth of invasion, lymph node metastasis and Duke＇s staging. And the expression of nm23-H1 was associated with depth of invasion, lymph node metastasis and Duke＇s staging. The expression of the three proteins in colorectal cancer tissues were possitively related. Conclusion The expressions of PTEN, nm23-H1 and Syk in colorectal cancer are related to carcinogenesis, devel- opment, invasion and metastasis. The joint detection of these three makers can be used to determine the biological behavior and prog- nosis of colorectal cancer.

关 键 词：大肠癌 抑癌基因 PTEN NM23-H1 SYK 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]