实时荧光PCR技术快速检测食品中的牛源成分  

Detection for bovine-derived ingredients in foods with real-time polymerase chain reaction method

作　　者：范丽丽[1] 李培[2] 丁洪流[2] 金萍[2] 傅春玲[1] 

机构地区：[1]苏州大学医学部公共卫生学院,江苏苏州215123 [2]苏州市产品质量监督检验所,江苏苏州215128

出　　处：《食品工业科技》2013年第12期65-67,85,共4页Science and Technology of Food Industry

基　　金：苏州市科技支撑计划项目(SS201126)

摘　　要：目的:建立基于实时荧光PCR技术的食品中牛源性成分快速检测方法。方法:以牛线粒体细胞色素b为目的基因,设计特异性引物和探针,通过特异性、灵敏性实验,及模拟混合肉样和市售肉制品检测,对该体系进行验证。结果:该牛源荧光PCR检测体系具有很好的特异性及灵敏性,可检测1pg牛源DNA的存在,对于各模拟肉类样品中掺杂的牛源性成分,其检测限低至0.5%,且经市售加工食品验证具有较好的应用能力。结论:所建立的牛引物探针体系具有特异性好、灵敏度高,快速高效等优点,可用于对食品中牛源性成分的掺假鉴别检测。Objective.This study was aimed to establish a real-time PCR assay for detection of bovine-derived ingredients in food. Methods .. Primers and Taqman probe of this assay were designed within bovine conservative regions of the mitochondrial cytochrome b（cyt b） gene. The specificity had been evaluated with no amplification on DNA from other meats while the detection sensitivity tested with meat mixtures containing various beef content. The assay was further applied to detect commercially available meat food for verifying its adaptability. Results：The assay was highly specific showing no amplification with other meats and could detect lpg of beef DNA. Applied to the DNA extracted from meat mixtures,it was possible to detect 0.5% beef spiked in other species. Conclusion.The system yields excellent results for identification of bovine derivatives in food products and it was a potentially reliable and suitable technique in routine food analysis for detection of bovine-derived ingredients in food.

关 键 词：实时荧光PCR 牛 线粒体细胞色素B基因 食品掺假 

分 类 号：TS207.3[轻工技术与工程—食品科学]