表面活性剂与β-环化糊精共同作用对放线菌羟基化维生素D_3的影响  

Effect of surfactant and β-cyclodextrin on vitamin D_3 Hydroxylased by Actinomyces

作　　者：王国盼[1] 苏宏飞[1] 韦春葵[1] 熊思驰[1] 黄飞 李丽[1] 黄时海[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]南宁高新区,广西南宁530007

出　　处：《食品工业科技》2013年第12期173-176,共4页Science and Technology of Food Industry

基　　金：南宁市科学研究与技术开发计划项目(20125193);梧州市科学研究技术开发计划项目(201201023)

摘　　要：放线菌CXX-10具有维生素D3的25位羟基化活性。通过研究Tween-80、聚乙二醇辛基苯基醚(Triton-100)、甘油、十二烷基硫酸钠(SDS)、十六烷基溴化铵(CTAB)等表面活性剂,与β-环化糊精共同作用对该菌25位羟基化维生素D3的影响,获得了较优的促发酵组合并优化了其反应条件:培养3d后添加0.04% Tween-80、0.5% β-环化糊精和VD3,pH为7.0,28℃,180r/min,发酵转化反应7d,25-(OH)D3产率达14.5mg/L。Actinomyces CXX-10 is capable of 25-hydroxylating of vitamin Ds. The effect of surfactant such as Tween-80, Triton-100, glycerin, sodium dodecyl sulfate （SDS）, hexadecyl trimethyl ammonium Bromide （CTAB） cooperate with β-cyclodextrin on productivity of 25-（OH）D3 was analysised in this paper. And then optimization of condition of most suitable combination of surfactant and β-cyclodextrin were promoted. The optimal conditions were as follows ：0.04% Tween-80 and 0.5% β-cyclodextrin and VD3 were added after 3d of culture, and other culture condition were 28℃, 180r/min,7d.

关 键 词：放线菌 25位羟化 β-环化糊精 表面活性剂 

分 类 号：TS201[轻工技术与工程—食品科学]