Characterization of MHC class Ⅱ A genes in Hainan Eld's deer(Cervus eldi hainanus)  

作　　者：LIU HongYi YANG HaiQiong GE YunFa 

机构地区：[1]The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Center for Gene Resources of Endangered Wildlife,College of Life Sciences,Zhejiang University

出　　处：《Chinese Science Bulletin》2013年第17期2148-2153,共6页

基　　金：supported by the National Natural Science Foundation of China (30970426);a special grant from the State Forestry Administration of China;the Fundamental Research Funds for the Central Universities of China

摘　　要：The major histocompatibility complex(MHC) genes play pivotal roles in the immune system of vertebrates against antigens.They are also significant indicators of genetic structure,and are vital to species-level population viability analyses and disease risk assessments.In this study,two DRA and two DQA sequences were isolated from Hainan Eld's deer(Cervus eldi hainanus) using rapid amplification of cDNA ends(RACE) and single-strand conformation polymorphism-heteroduplex(SSCP-HD) analysis.Nucleotide sequence analysis revealed large differences between the two DQA sequences,especially in their exon 2 regions,but only minimal differences between the variants of the DRA gene.Comparison of the predicted amino acid sequences of the Ceel-MHC class Ⅱ A variants with those from six other species revealed that these molecules share high homology among ruminants.A phylogenetic tree of four class Ⅱ A sequences from Hainan Eld's deer and the other species placed the newly identified DQA and DRA genes on two distinct branches(100%-supportively),and further divided the two DQA sequences into 98%-supportive DQA1 and 99%-supportive DQA2 clusters,respectively.Therefore,this study identified monomorphic Ceel-DQA1 and Ceel-DQA2 genes,and one dimorphic Ceel-DRA gene from Hainan Eld's deer.The major histocompatibility complex （MHC） genes play pivotal roles in the immune system of vertebrates against antigens. They are also significant indicators of genetic structure, and are vital to species-level population viability analyses and disease risk assessments. In this study, two DRA and two DQA sequences were isolated from Hainan Eld＇s deer （Cervus eldi hainanus） using rapid amplification of cDNA ends （RACE） and single-strand conformation polymorphism-heteroduplex （SSCP-HD） analysis. Nucleotide sequence analysis revealed large differences between the two DQA sequences, especially in their exon 2 regions, but only minimal differences between the variants of the DRA gene. Comparison of the predicted amino acid sequences of the Ceel-MHC class II A variants with those from six other species revealed that these molecules share high homology among ruminants. A phylogenetic tree of four class II A sequences from Hainan Eld＇s deer and the other species placed the newly identified DQA and DRA genes on two distinct branches （100%-supportively）, and further divided the two DQA sequences into 98%-supportive DQA1 and 99%-supportive DQA2 clusters, respectively. Therefore, this study identified monomorphic CeeI-DQA1 and CeeI-DQA2 genes, and one dimorphic Ceel-DRA gene from Hainan Eld＇s deer.

关 键 词：海南坡鹿 MHC 基因 核苷酸序列分析 主要组织相容性复合体 快速扩增CDNA末端 种群生存力分析 单链构象多态性 

分 类 号：Q78[生物学—分子生物学]