The mitochondrial genome encodes abundant small noncoding RNAs  

作　　者：Seungil Ro Hsiu-Yen Ma Chanjae Park Nicole Ortogero Rui Song Grant W Hennig Huili Zheng Yung-Ming Lin2 Loredana Moro Jer-Tsong Hsieh wei Yant 

机构地区：[1]Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA [2]Department of Urology, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan 70403, China [3]Institute of Biomem- branes and Bioenergetics, National Research Council (CNR), Bari 70123, Italy [4]Department of Urology, Southwestern Medical Center, University of Texas, Dallas, TX 75235, USA

出　　处：《Cell Research》2013年第6期759-774,共16页细胞研究（英文版）

摘　　要：Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell.Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell.

关 键 词：MITOCHONDRIA small noncoding RNAs RNA interference TRANSCRIPTION gene expression circular genome 

分 类 号：Q943[生物学—植物学] Q522