Electrochemical biosensors and logic devices based on aptamers  

作　　者：Zuo Xiaolei Lin Meihua Fan Chunhai 

机构地区：[1]Laboratory of Physical Biology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences

出　　处：《Engineering Sciences》2013年第3期76-79,共4页中国工程科学（英文版）

基　　金：100 Talents Program of Chinese Academy of Sciences;National Key Basic Research Program of China ("973"Program) (No. 2012CB932600)

摘　　要：Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers have excellent properties such as cost-effective,stable,easy for synthesis and modification. In recent years,electrochemistry plays an important role in biosensor field because of its high sensitivity,high stability, fast response and easy miniaturization. Through the combination of these two technologies and our rational design,we constructed a series of biosensors and biochips that are simple,fast,cheap and miniaturized. Firstly,we designed an adenosine triphosphate (ATP) electrochemical biosensor based on the strand displacement strategy. We can detect as low as 10 nmol/L of ATP both in pure solution and complicated cell lysates. Secondly,we creatively split the aptamers into two fragments and constructed the sandwich assay platform only based on single aptamer sequence. We successfully transferred this design on biochips with multiple micro electrodes (6×6) and accomplished multiplex detection. In the fields of biochips and biocomputers,we designed several DNA logic gates with electric (electrochemical) signal as output which paves a new way for the development of DNA computer.

关 键 词：APTAMER BIOSENSOR BIOCHIP bio-computer logic device 

分 类 号：Q74[生物学—分子生物学] TP212.3[自动化与计算机技术—检测技术与自动化装置]