杏鲍菇工厂化栽培条件的响应面优化研究  

Study on Optimization of the Cultural Conditions Using Response Surface Analysis for Factory Cultivation of Pleurotus eryngii

作　　者：林凯[1] 李宗堂 肖奎 李可[1] 向文良[1] 张庆[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]四川榕珍菌业有限公司,四川成都611733

出　　处：《安徽农业科学》2013年第10期4602-4605,共4页Journal of Anhui Agricultural Sciences

基　　金：成都市科技攻关计划(10GGZD21NC-268)

摘　　要：[目的]建立杏鲍菇工厂化栽培条件的理论预测模型,提高其生物学转化率。[方法]运用Plackett-Burman试验设计,对影响杏鲍菇生物转化率的主要影响因子进行筛选,通过Box-Behnken试验设计和响应面优化分析影响因素,得到最佳栽培条件。[结果]Plackett-Burman试验表明,影响杏鲍菇生物转化率的主要因子为温度、湿度和含水量;Box-Behnken试验得最佳栽培条件为:温度15.13℃,湿度91.02%,含水量66.00%,优化栽培参数后,杏鲍菇的生物转化率达到92.10%,比优化前提高了6.40%。[结论]响应面分析法有效提高了杏鲍菇生物转化率,可为杏鲍菇的工厂化高效高产栽培提供参考。[Objective] To establish the theoretical prediction model of the cultural conditions for factory cultivation of Pleurotus eryngii,so as to improve its biotransformational rate.[Method] Plackett-Burman experiment design was used to screen out the key factors which may influence factors affecting the biotransformational rate of Pleurotus eryngii.The Box-Behnken experiment design and response surface analysis were used to determine the optimal cultural conditions.[Result] The results of Plackett-Burman experiment showed that the biotransformational rate of Pleurotus eryngii was mainly influenced by temperature,humidity and water content.The optimum cultural conditions that was obtained from Box-Behnken experiment were as follows: temperature of 15.13 ℃,humidity of 91.02% and water content of 66.00%.The biotransformational rate of Pleurotus eryngii was up to 92.10% which improved 6.40% than the experiment before optimization.[Conclusion] The response surface design effectively improved the biotransformational rate of Pleurotus eryngii,which could provide reference for factory cultivation Pleurotus eryngii with high-efficiency and high-yield.

关 键 词：杏鲍菇 栽培条件 生物转化率 响应面优化 

分 类 号：S646.14[农业科学—蔬菜学]