转座酶对piggyBac转座子在弓形虫内转座活性的影响  

Effect of Transposase on the Transposition Activity of piggyBac Transposon Transfected into Toxoplasma gondii

作　　者：宋鑫帅[1] 魏峰[1] 张莹光[1] 曹莉莉[2] 刘全[2] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]军事医学科学院军事兽医研究所.吉林省人兽共患病预防与控制重点实验室,长春130122

出　　处：《中国寄生虫学与寄生虫病杂志》2013年第3期244-244,F0003,共2页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.31072127,30972178,31001057)~~

摘　　要：为研究转座酶(PBase)对转座子piggyBac在弓形虫细胞内转座效率的影响,将转座酶质粒Toxo-PBase和带红色荧光蛋白(RFP)的转座子质粒PB-Toxo-RFP共同电转染刚地弓形虫RH株速殖子,流式细胞仪检测转染后的弓形虫红色荧光蛋白的转座效率。结果显示,PB-Toxo-RFP和Toxo-PBase共转染组转座效率为73%,PB-Toxo-RFP转染组转座效率为43%,前组显著高于后组(P<0.01),表明转座酶能够提高piggyBac转座子在弓形虫细胞内的转座效率。To determine the transfection efficiency about PBase to piggyBac transposon in transfecting to Toxo- plasma gondii, T. gondii RH strain tachyzoites were transfected with plasmid PB-Toxo-RFP which was expressed piggyBac transposon with a red fluorescent protein and Toxo-PBase plasmid which is a transposable enzyme. T. gondii tachyzoites were transfected with PB-Toxo-RFP plasmid alone as control group. The expression of red fluorescent protein was detected by flow cytometry at 24 h after transfection. The transposition efficiency in PB-Toxo-RFP＋Toxo-PBase group and PB-Toxo- RFP group was 73% and 43%, respecitvely （P〈0.01）. It suggests that the PBase transposase can improve the transfection efficiency of piggyBac transposon in T. gondii tachyzoites.

关 键 词：PIGGYBAC转座子 转座酶 转座效率 刚地弓形虫 

分 类 号：R382.5[医药卫生—医学寄生虫学]