小鼠乳腺癌辐射敏感细胞SX-9的ku基因突变与辐射敏感性研究  

ku gene mutation of radiosensitive mouse mammary carcinoma derived cell line SX\|9 \

作　　者：江国春[1] 袁丽珍[1] 魏康[1] 郭学敏 张雪峰[1] 贾向旭[1] 

机构地区：[1]北京放射医学研究所,100850

出　　处：《中华放射医学与防护杂志》2000年第4期242-244,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金!资助项目 (3990 0 0 31)

摘　　要：目的　研究辐射敏感小鼠乳腺癌细胞SX 9的ku70、ku80基因型 ,初步探讨ku基因突变对其辐射敏感性的影响。方法　RT PCR克隆并鉴定ku70 /ku80全长基因 ,并对其全长进行全自动测序。脂质体转染正常人全长ku80cDNA至SX 9细胞 ,克隆形成比较转染了正常ku80基因的SX 9与SX 9以及SR 1细胞存活率。结果　SX 9细胞ku70基因正常 ,而其ku80存在基因突变 ,转染了正常人全长ku80cDNA的SX 9细胞能部分重建其对γ射线的存活率。结论　SX 9细胞ku基因发生突变 。Objective\ To investigate the ku gene status of radiosensitive cell line SX\|9 and the effects of ku80 gene mutation on radiosensitivity of SX\|9. Methods\ After comparing the ku70/80 gene status of SX\|9 to SR\|1 with molecular cloning,the survival fraction of SX\|9 transfected with wild\|type human ku80,SX\|9 and SR\|1 was investigated by clonogenic assay. Results\ Radiosensitive mouse mammary carcinoma\|derived cell line SX\|9 bore mutation in ku80 gene,and its survival fraction after γ\|ray irradiation could be restored by transfection of wild\|type human ku80 gene. Conclusions\ There are two mutation sites in ku80 gene in radiosensitive mouse mammary carcinoma\|derived cell line SX\|9,which could be the cause of its deficiency in repair of DNA double strand breaks.\;

关 键 词：乳腺癌 辐射敏感性 SX-9 ku基因 基因突变 

分 类 号：R737.9[医药卫生—肿瘤]