双波长分光光度法测定烟草中的直链淀粉和支链淀粉  

Determination of Contents of Amylose and Amylopectin in Tobacco by Dual Wavelength Spectrophotometry

作　　者：王文超[1] 贺帆[1] 宋朝鹏[1] 王涛[1] 董艳辉[1] 杨彦明[1] 娄元菲[1] 宫长荣[1] 

机构地区：[1]河南农业大学烟草学院,郑州市文化路95号450002

出　　处：《烟草科技》2013年第6期50-52,63,共4页Tobacco Science & Technology

基　　金：国家烟草专卖局资助项目"密集烘烤工艺优化研发"(3300806156)

摘　　要：根据双波长比色原理,选择直链淀粉的测定波长为512和596 nm,支链淀粉的测定波长为560和695 nm,依据回归方程求出直链和支链淀粉的含量。结果表明:用双波长分光光度法测定直链淀粉和支链淀粉的含量结果准确,适用于批量分析。Determining the contents of amylose and amylopectin in tobacco with dual-wavelength spectrophotometry was new, although it has been widely applied to other crops. The measurement wavelengths of 512 and 596 nm for amylose and 560 and 695 nm for amylopectin were selected according to the principle of dual wavelength colorimetric, and the contents of amylose and amylopectin were calculated based on regression equations. The results showed that the contents of amylose and amylopectin in tobacco determined with dual-wavelength spectrophotometry were accurate, and the method was suitable for the determination of amylose and amylopectin in batches of tobacco samples.

关 键 词：双波长分光光度法 直链淀粉 支链淀粉 烟草 含量测定 

分 类 号：TS411.1[农业科学—烟草工业]