嗜热脂肪芽孢杆菌高温蛋白酶的产生条件及酶学性质  

PRODUCTION AND SOME PROPERITIES OF A THERMOPHILIC PROTEASE FROM BACILLUS STEAROTHERMOPHILUS WF146

作　　者：唐兵[1] 周林峰 陈向东[1] 戴玄[1] 彭珍荣[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《微生物学报》2000年第2期188-192,共5页Acta Microbiologica Sinica

基　　金：武汉市晨光计划资助项目!( 985 0 0 30 77)

摘　　要：对嗜热脂肪芽孢杆菌(Bacillusstearothermophilis)WF146的产蛋白酶的条件进行了研究,在58℃条件下,WF146在pH值为75的Fd培养基中振荡发酵培养48h后,发酵液中高温蛋白酶产量可达600u/mL以上。对该酶性质的研究表明,酶分子量为34kD,最适作用pH为80,最适作用温度为80℃,具有良好的pH稳定性及热稳定性。Ca2+对该酶的稳定性具有重要影响,PMSF、DFP及IAA能强烈抑制酶活力。The factors affecting Bacillus stearothermophilus WF146 for thermophilic protease producing have been investigated, more than 600 units of enzyme in 1mL of fermented culture could be achieved under suitable condition. The protease had a molecular weight around 34kD estimated by SDS PAGE, and functioned optimally at pH8 0 and 80℃, respectively. In addition, the enzyme exhibited high temperature tolerance and was stable at a wide range of pH, and Ca 2+ played a key role for the stability of the enzyme. While the protease activity of the enzyme was strongly inhibited by PMSF, DFP and IAA, and was not affected by DTT.

关 键 词：嗜热脂肪芽孢杆菌 高温蛋白 产酶条件 酶性质 

分 类 号：Q936[生物学—微生物学]