葡萄糖异构酶的生物工程研究进展  

Progress in Biological Engineering of D-Glucose Isomerase .

作　　者：朱国萍[1] 程阳 宫春红 徐冲[1] 

机构地区：[1]中国科学技术大学生命科学学院,合肥230026

出　　处：《生物化学与生物物理进展》2000年第2期127-131,共5页Progress In Biochemistry and Biophysics

基　　金：国家"8 63"高技术项目!( 13 0 13 0 2 0 4)

摘　　要：D 葡萄糖异构酶 (GI)能分别催化D 葡萄糖和D 木糖异构为D 果糖和D 木酮糖 .它是工业上生产高果糖浆的关键酶 .在GI负氢离子转移机制中 ,其主要特征是底物的开环、通过C2 至C1间负氢离子转移的GI异构化作用、以及产物的闭环 .GI基因已在同源、异源宿主中获得克隆、测序和超量表达 .经过蛋白质工程改造的GI将是未来最重要的工业用酶之一 .D glucose isomerase(GI) can isomerize D glucose and D xylose into D fructose and D xylulose ,respectively. It is a crucial enzyme in the production of high fructose corn syrup on industrial scale. In the hydride shift mechanism proposed for GI,the main features are ring opening of the substrate,isomerization of GI via a hydtride shift from C 2 to C 1,and ring closure of the product. GI gene has been cloned,sequenced and overexpressed in the homologous and heterogous hosts. GI whose properties have been improved by protein engineering may be one of the most important industrial enzymes of the future.

关 键 词：葡萄糖异构酶 高果糖浆 蛋白质工程 空间结构 

分 类 号：Q558[生物学—生物化学] TQ93[化学工程]