腺相关病毒介导基因组来源的基因转移与整合  

Stable Integration of Genomic Gene of Interest in Mammalian Cells Following Adeno-associated Virus Vector Mediated Gene Transfer.

作　　者：李斌[1] 刘德培[1] 王晶 董文吉 郭志晨 夏薇 梁植权 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所,医学分子生物学国家重点实验室,北京100005

出　　处：《生物化学与生物物理进展》2000年第2期189-192,共4页Progress In Biochemistry and Biophysics

基　　金：863计划项目资助

摘　　要：构建一个带 β 珠蛋白基因组序列的腺相关病毒载体AV5 3HS2Δβ2Neo .经包装成重组腺相关病毒后 ,转导红系细胞 .DNA印迹证实包含红系增强子、β 珠蛋白基因和筛选标志基因的前病毒基因组完整整合于红系细胞基因组中 .An adeno associated viral vector containing erythroid enhancer(HS2), genomic β globin gene and neomycin resistance gene was constructed, then packaged into recombinant AAV virions. Southern analyses showed that recombinant provirus genome steadily integrated into erythroid cells via virus transduction. It was suggested that an adeno associated virus could mediate stable integration of genomic gene of interest in mammalian cells.

关 键 词：腺相关病毒载体 基因组 基因转移 整因整合 

分 类 号：R394.8[医药卫生—医学遗传学] Q78[医药卫生—基础医学]