阿维菌素高产菌株的选育及阿维菌素B_1的鉴定  

Selection of High Avermectins Producing Strain and Identification of Avermectin B_1

作　　者：宋渊[1] 曹贵明[1] 陈芝[1] 李季伦[1] 

机构地区：[1]中国农业大学生物学院微生物学系,北京100094

出　　处：《生物工程学报》2000年第1期31-35,共5页Chinese Journal of Biotechnology

基　　金：国家攻关课题!( 96 C0 1 0 2 0 3 )

摘　　要：自阿维链霉菌(StreptomycesavermitilisATCC31272)中分离出了3种不同类型的菌株,其中只有产灰色孢子的菌株能产生阿维菌素(Avermectins),摇瓶发酵单位约100μg/mL。经高频电子流诱变和对发酵培养基的改进,选育出Sa76菌株,其摇瓶发酵单位可达1000μg/mL。从其菌丝体中提取纯化了阿维菌素B1晶体,其紫外吸收光谱、红外吸收光谱、核磁共振谱(1HNMR和13CNMR)和质谱与国外报道的一致。Sa76菌株又经2次亚硝基胍诱变,筛选出发酵单位2000μg/mL以上的Sa768菌株。在此基础上,再次用亚硝基胍对Sa768菌株进行了诱变,获得Sa769菌株,结合发酵条件的优化,其发酵单位可高达3500～4000μg/mL。Three types of colony,powdery gray,white and bald,were isolated from Streptomyces avermitilis ATCC31272.Among them,only the powdery grey one produces avermectins.Sa 76 strain was selected from the powdery grey strain by mutation with high energy electric flow,and its avermectins titer attainde 100μg/mL in shaking flask.Avermectin B 1 was extracted and purified from the mycelia of Sa 76,and identified by UV,IR, 1H NMR, 13 C NMR and mass spectra.After Sa 76 strain was treated twice with NTG,a strain named Sa 76 8 was selected with avermectins titer over 2000μg/mL.The Sa 76 8 strain was treated with NTG once again,and a high avermectins producing strain named as Sa 76 9 with avermectins titer up to 3500～4000μg/mL was selected.

关 键 词：阿维链霉菌 阿维菌素B1 菌株选育 发酵 

分 类 号：Q344.13[生物学—遗传学]