三乙胺离子对HPLC法测定乳制品中三聚氰胺的质量分数  

Determination of melamine in dairy materials by RP-IP-HPLC

作　　者：张艳萍[1] 尤玉如[1] 

机构地区：[1]浙江科技学院生物与化学工程学院,杭州310012

出　　处：《中国乳品工业》2013年第6期43-45,共3页China Dairy Industry

基　　金：浙江省分析测试基金项目(2007F70004)

摘　　要：建立了高效液相色谱法检测乳制品中三聚氰胺的方法。样品由质量分数为1％三氯乙酸一乙腈超声提取．加入蛋白沉淀剂进行纯化。色谱柱zoRBAxSB—Aq（250mmx4．6mm，5μm），流动相为磷酸一三乙胺溶液，流速为0．6mL／min检测波长5-240nm，回收率为86．4％-90．8％，相对标准偏差在0．69％2．11％之间。An analytical method for determination for melarrfine in dairy materials by HPLC was developed. Samples were extracted by blending with 10% TCA and acetonitrile. The extract was purified by precipitating protein. The HPLC conditions： ZORBAX SB-Aq （250 min×4.6 mm, 5 μm）, phosphoric acid-triethylamine as mobile phase and UV detection at 240 nm. The average recoveries were 86.4~90.8% and RSD was 0.69-2.11%.

关 键 词：三聚氰胺 乳制品 高效液相色谱法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]