BP1基因在甲状腺癌组织中的表达及其临床意义  

Clinical significance of BP1 gene expression in human thyroid cancer

作　　者：王奇龙[1] 孙学军[1] 王林古[2] 张仕运[1] 成亮[1] 

机构地区：[1]西安交通大学医学院第一附属医院普外科,西安710061 [2]西安交通大学医学院第一附属医院耳鼻咽喉头颈外科,西安710061

出　　处：《中华内分泌外科杂志》2013年第3期218-220,共3页Chinese Journal of Endocrine Surgery

基　　金：陕西省科学技术研究发展计划项目（2010k01-136）

摘　　要：目的检测BP1在甲状腺癌组织中的表达，探讨其与甲状腺癌临床病理因素的关系。方法运用原位杂交及免疫组化方法检测60例甲状腺癌组织（研究组）和20例正常甲状腺组织（对照组）中的BP1表达。结果原位杂交方法检测BP1mRNA阳性率在研究组中为78．3％（47／60），对照组中为20％（4／20），BP1基因在2组的表达差异有统计学意义（x。=22．09，P〈0．05）；甲状腺癌各病理类型的阳性表达率分别为乳头状癌81．7％（40／49）、滤泡状癌85．7％（6／7）、髓样癌25．0％（1／4）；髓样癌分别与乳头状癌、滤泡状癌之间BP1mRNA的表达差异有统计学意义（，=4．21，P〈0．05）；免疫组化检测研究组中BP1蛋白阳性率为93．3％（56／60），对照组中BP1蛋白阳性率为10．0％（2／20），2组间BP1的表达差异有统计学意义（χ2=52．25，P〈0．05）。国际抗癌联盟（UICC）Ⅰ～Ⅱ期BP1mRNA阳性率90．9％（40／44），Ⅲ～Ⅳ期43．8％（7／16），差异有统计学意义（χ2=12．72，P〈0．05）。但BP1mRNA表达与性别、年龄及淋巴结转移无相关性。结论BP1在甲状腺癌组织中的表达显著上调；BP1基因的表达与其UICC分期、病理学类型有关，而与年龄、性别及淋巴转移无关。Objective To detect the expression of BP1 gene in thyroid cancer and its relationship with clinicopathological features of thyroid cancer. Methods BP1 gene expression in 60 cases of thyroid cancer tis- sues and 20 cases of normal thyroid tissues were detected by in situ hybridization and immunohistoehemistry. Results The positive expression rate of BP1 mRNA was 78.3% （47/60） in the 60 cases of thyroid cancer tissues while it was 20% （4/20） in the 20 cases of normal thyroid tissues detected by in situ hybridization. The difference had statistical significance （P 〈 0. 05 ）. Of the 3 pathological types of thyroid cancer, the positive expression rate of papillary carcinoma was 81.6% （40/49）, follicular carcinoma 85.7% （6/7）, and medullary carcinoma 25.0% （1/4）. The expression of BP1 mRNA had statistical difference between medullary carcinoma and other pathological types like papillary carcinoma and follicular carcinoma （ P 〈 0. 05 ）. The positive expression rate of BP1 protein was 93.3 % （56/60）in the 60 cases of thyroid cancer tissues while it was 10. 0% （2/20）in the 20 cases of normal thyroid tissues detected by immunohistochemistry. The difference had statistical significance（P 〈 0. 05）. Conclusion BP1 gene expression is up-regulated in human thyroid cancer and it is related to tumor stage and pathological type but not related to patients＇ age, sex or lymph node metastasis.

关 键 词：甲状腺癌 BP1基因 原位杂交 免疫组化 

分 类 号：R736.1[医药卫生—肿瘤]