超声波辅助-DMC萃取食用油中苯并(a)芘及其GC/MS分析  

Ultrasound assisted extraction with environmental friendly solvent for the determination of B(a) p in edible oil by GC/MS

作　　者：王珍[1] 王琳琳[1] 陈小鹏[1] 龙柳锦[1] 庄连蝶[1] 

机构地区：[1]广西大学化学化工学院,广西石化资源加工及过程强化技术重点实验室,广西南宁530004

出　　处：《广西大学学报（自然科学版）》2013年第3期538-543,共6页Journal of Guangxi University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(31060102);广西自然科学基金资助项目(2013GXNSFAA019050);广西石化资源加工及过程强化技术重点实验室主任基金(Z001)

摘　　要：为了准确、快速检测食用油中苯并(a)芘的含量,建立了食用油中苯并(a)芘的超声波辅助-绿色溶剂萃取及GC/MS联用技术检测方法,其检出限和定量限分别为0.017μg/L和0.053μg/L,加标回收率大于95%,日内和日间相对标准偏差分别为1.32%～1.76%和2.46%～3.17%,表明该方法符合痕量分析要求。实验考察了萃取剂种类、配比、用量、超声波功率、温度和时间对苯并(a)芘萃取的影响,获得了较优的萃取工艺条件:ACN-DMC配比(V/V)7∶3,萃取剂用量25 mL,超声功率300 W,温度20℃,时间10 min/次,萃取两次。采用该方法测定38个食用油样中苯并(a)芘含量,结果表明,其中30个不同品牌的新鲜食用油苯并(a)芘含量范围为0.09～8.17μg/kg;8个油炸食用油苯并(a)芘含量范围为6.03～11.73μg/kg。本方法耗时短且减少了有毒溶剂的使用,为食用油中含有的苯并(a)芘分析检测提供了一种科学简捷的方法。Abstract： To quickly and accurately analyze B （a）p in edible oils, an ultrasonic assisted extraction with environmental friendly solvent using gas chromatography-mass spectrometry （GC-MS）method was developed. The limits of detection and quantization obtained were 0. 017 p,g/L and 0. 053μg/L respectively, and the recovery rate was higher than 95% and the relative standard deviations were 1.32% ~ 1.76% for inter-day and 2. 46% -3.17% for intra-day, demonstrating the proposed method was feasible and effective. Optimizing the conditions of ultrasound assisted extraction such as extractant, ration, dosage, ultrasonic power, temperature and time, an optimum method was deve- loped. The levels of B（a） p by using this method ranged from 0. 09 μg/kg to 8. 17μg/kg in 30 flesh edible oil samples, from 6.03 μg/kg to 11.73 μg/kg in 8 frying oil samples. The development of this method provided a scientific and simple method for determination of B （a） p in less time and solvent.

关 键 词：苯并(a)芘(B(a)p) 食用油 碳酸二甲酯(DMC) 气相色谱-质谱(GC MS) 超声波 

分 类 号：O625.1[理学—有机化学]