Proteomic Identification of Differentially Expressed Proteins in Vaccaria segetalis-treated Dairy Cow Mammary Epithelial Cells  

作　　者：Liu Rong Li Qing-zhang Huang Jian-guo Lu Li-min Gao Xue-jun 

机构地区：[1]Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University

出　　处：《Journal of Northeast Agricultural University(English Edition)》2013年第2期24-31,共8页东北农业大学学报（英文版）

基　　金：Supported by Major State Basic Research Development Program of China (973 Program, 2011CB100804)

摘　　要：To investigate the potential effects of Vaccaria segetalis, we employed the proteomic approach on the dairy cow mammary gland epithelial cells （DCMECs）. A total of 35 differentially expressed nuclear proteins were visualized by 2-DE and silver nitrate staining. Of these, five proteins also displayed significant expression changes upon treatment of the water decoction from Vaccaria segetalis, and such alterations were further confirmed by RT-PCR. Together, at both the mRNA and protein levels, the water decoction from Vaccaria segetalis increased the expression of proteins ethylmalonic encephalopathy 1 （ETHE1）, vesicle amine transport protein 1 homolog （VAT1）, parkinson disease protein 7 homolog （Protein DJ-1）, proteasome subunit alpha type-2 （PSMA2） and SUMO-activating enzyme subunit 1 （SAE1）. This study would enable a better understanding of the molecular mechanisms underlying this water decoction effects at the protein level.To investigate the potential effects of Vaccaria segetalis, we employed the proteomic approach on the dairy cow mammary gland epithelial cells （DCMECs）. A total of 35 differentially expressed nuclear proteins were visualized by 2-DE and silver nitrate staining. Of these, five proteins also displayed significant expression changes upon treatment of the water decoction from Vaccaria segetalis, and such alterations were further confirmed by RT-PCR. Together, at both the mRNA and protein levels, the water decoction from Vaccaria segetalis increased the expression of proteins ethylmalonic encephalopathy 1 （ETHE1）, vesicle amine transport protein 1 homolog （VAT1）, parkinson disease protein 7 homolog （Protein DJ-1）, proteasome subunit alpha type-2 （PSMA2） and SUMO-activating enzyme subunit 1 （SAE1）. This study would enable a better understanding of the molecular mechanisms underlying this water decoction effects at the protein level.

关 键 词：PROTEOMICS 2-DE Vaccaria segetalis DCMECs RT-PCR 

分 类 号：Q78[生物学—分子生物学] Q81