Adsorption Reaction Dynamics of Systems Lysozyme and Nanodiamond/Nanosilica at pH=7-13  

溶菌酶-蛋白质与奈米钻石／奈米硅土之吸附反应动力学

作　　者：赵(吴)为克 

机构地区：[1]国立高雄应用科技大学化学工程与材料工程学系,高雄80782 [2]维克人文科技育成顾问公司,D-33602比勒费尔德

出　　处：《Chinese Journal of Chemical Physics》2013年第3期295-302,I0003,I0004,共10页化学物理学报（英文）

摘　　要：Adsorption reactions between surfaces of nanodiamond and nanosilica with diameter of 100 nm prepared as suspension solutions of 0.25μg/μL and lysozyme molecule with different concentrations of 7 mmol/L PPBS at pH=7, 9, 11, and 13 have been investigated by fluores- cence spectroscopy. Adsorption reaction constants and coverages of lysozyme with different concentrations of 0-1000 nmol/L under the influences of different pH values have been ob- tained. Helicities and conformations of the adsorbed lysozyme molecules, free spaces of every adsorbed lysozyme molecule on the surfaces of nanopartieles at different concentrations and pH values have been deduced and discussed. The highest adsorption capabilities for both sys- tems and conformational efficiency of the adsorbed lysozyme molecule at pH=13 have been obtained. Lysozyme molecules can be prepared, adsorbed and carried with optimal activity and helicity, with 2 and 10 mg/m2 on unit nanosurface, 130 and 150 mg/g with respect to the weight of nanoparticle, within the linear regions of the coverages at around 150-250 nmol/L and four pH values for nanodiamond and nanosilica, respectively. They can be prepared in the tightest packed form, with 20 and 55 mg/m2, 810-1680 and 580-1100 mg/g at threshold concentrations and four pH values for nanodiamond and nanosilica, respectively.

关 键 词：Protein adsorption Interracial reaction dynamics Fluorescence spectroscopy Single molecular spectroscopy BIOCHIP PROTEOMICS 

分 类 号：O6[理学—化学]