功能微粒材料的制备及其细胞作用研究(英文)  

Advanced Particulate Biomedical Materials and Their Interactions with Cells

作　　者：毛峥伟[1] 张元洪[1] 李慧英[1] 仝维鋆[1] 高长有[1] 

机构地区：[1]教育部高分子合成与功能构造重点实验室浙江大学高分子科学与工程学系,杭州310027

出　　处：《化学进展》2013年第7期1061-1070,共10页Progress in Chemistry

基　　金：supports by the Natural Science Foundation of China (No.51120135001,20934003);the National Basic Research Program of China (No.2011CB606203);the Ph.D. Programs Foundation of Ministry of Education of China (No.20100101120034,20110101130005);“Qianjiang” outstanding researcher funding of Zhejiang Province (No.J20110541);Department of Education of Zhejiang Province (No.Z201018687)

摘　　要：随着纳米科学的迅速发展,越来越多的微粒材料被设计和制备出来,并应用于生物医学领域,取得了令人瞩目的进展和成就。近年来,我们课题组发展了一系列具备可控表面化学与结构、特定刺激响应性能和可控装载与释放性能的微粒材料,尤其是基于层层自组装的中空微胶囊。同时,我们也致力于研究微粒材料与细胞的相互作用,阐明其物理化学性质对细胞内吞和细胞功能的影响。通过这些研究,我们希望进一步建立功能性微粒材料的生物学功能优化的规律,推动其在生物医学领域的应用。Along with the rapid development of micro and nanotechnology, the colloidal particles are taking more and more important roles in biological field as detective probes for disease diagnosis and drug carriers for sustained release and targeted delivery. On one hand, to fulfill the demands of these biological applications, various novel particulate materials with finely tuned surface chemistry and morphology, well defined stimuli responsiveness as well as adjustable substance loading and release properties have been developed in our lab. On the other hand, efforts are made to understand the cellular uptake of particles, and thereby the influence on cell functions, which is mandatory for their biomedical applications. In this mini-account, we systematically summarize our efforts to prepare and functionalize the particulate materials, especially the hollow microcapsules fabricated by the layer-by-layer （LbL） assembly of oppositely charged polyelectrolytes on colloidal templates, followed by core removal. The influences of physiochemical properties of these colloidal particles on the cellular uptake, intracellular distribution and transportation are then introduced. Their impacts on cytotoxicity and subsequent cell functions, especially on cell mobility and phenotype, are also discussed.

关 键 词：胶体微粒 微胶囊 层层组装 细胞内吞 细胞功能 

分 类 号：O63[理学—高分子化学] Q28[理学—化学]