南京及深圳两地女性宫颈HPV感染基因谱的比较研究  

A comparative research on gene spectrum of cervical cells in women infected with HPV in Nanjing and Shenzhen regions

作　　者：朱岩[1,2] 张晓甦[3] 耿建祥[3] 于红娟[3] 王宏景[3] 柏敏[3] 张劲松[3] 龙秀荣[3] 赵雪[3] 

机构地区：[1]南方医科大学附属深圳妇幼保健院 [2]深圳市妇幼保健院检验科,广东深圳518028 [3]南京中医药大学第三附属医院病理科江苏省HPV协助组,江苏南京210001

出　　处：《国际检验医学杂志》2013年第12期1511-1513,共3页International Journal of Laboratory Medicine

基　　金：江苏省南京市卫生局中医专项资助项目(2009-92)

摘　　要：目的比较南京及深圳两地女性宫颈细胞中23种人乳头瘤病毒(HPV)感染的基因型分布情况及其感染的差异性。方法宫颈细胞标本取自南京市扬子石化集团公司的3678例及深圳市妇幼保健院体检的2915例女性,采用基因扩增结合基因芯片技术对6593例宫颈细胞标本进行23种HPV基因型别的检测,并对其结果进行分析。结果 6593例宫颈细胞标本中共检出HPV感染者共1002例,HPV总感染率为15.20%(1002/6593),南京地区总的HPV感染率为10.79%(397/3678),深圳地区总的HPV感染率为20.76%(605/2915);HPV一重感染率南京地区为9.11%(335/3678),深圳地区为15.09%(440/2915);HPV多重感染率南京地区为1.69%(62/3678),深圳地区为5.66%(165/2915)。结论深圳地区HPV总的、一重和多重感染率都明显高于南京地区。Objective To compare the differences of genotype distribution and infection of 23 kinds of human papillomavirus（HPV） in women cervical cells in Nanjing and Shenzhen regions.Methods Cervical cell samples were obtained from 3 678 women worked in Nanjing Yangzi Petrochemical Corporation and 2 915 women who had undertaken physical examination in Shenzhen Women Child Health Hospital.Genetic amplification combined with gene chips technology was employed to detect genotypes in 23 kinds of HPV in 6 593 samples of cervical cells.Results 1 002 in 6 593 samples of cervical cells were detected to be infected with HPV with overall infection rate of 15.20%（1 002/6 593）.The overall HPV infection rate in Nanjing was 10.79%（397/3 678） and 20.76%（605/2 915） in Shenzhen.The single HPV infection rate in Nanjing was 9.11%（335/3 678） and 15.09%（440/2 915） in Shenzhen.The multiple HPV infection rate was 1.69%（62/3 678） and 5.66%（165/2 915） in Shenzhen..Conclusion The overall,single and multiple HPV infection rates in Shenzhen are all markedly higher than those in Nanjing.

关 键 词：宫颈 人乳头瘤病毒 基因谱 基因分型 

分 类 号：R737.33[医药卫生—肿瘤]