高效液相色谱法测定保健食品中水飞蓟宾含量的方法研究  

Determination of silybin in health food by high performance liquid chromatography

作　　者：刘家阳[1] 高岩[1] 贾宏欣[1] 王美菡[1] 

机构地区：[1]辽宁省疾病预防控制中心,辽宁沈阳110005

出　　处：《中国卫生检验杂志》2013年第6期1422-1423,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立保健食品中水飞蓟宾的高效液相色谱测定方法。方法:采用WATERS Xterra RP18(4.6×250 mm,5μm)色谱柱分离;流动相为甲醇:0.5%冰醋酸=48:52(V/V);流速1.00 ml/min;2487紫外检测器287 nm测定。结果:水飞蓟宾在1.0μg/ml～50μg/ml时,浓度与峰面积呈良好的线形关系(r=0.9998),检出限为0.20μg/ml,加标回收率在94.3%～98.0%之间,相对标准偏差RSD在2.40%～2.66%之间。结论:该方法简便、快速、准确,适用于保健食品中水飞蓟宾含量的测定。Objective： To establish a HPLC method for the determination of silybin in health food. Methods ： The separation was carried out on a Xterra RP18 （4.6 × 250mm,5 um） column with 2487 UV detector at 287nm , the mobile phase were methanol and 0.5% acetic acid（48：52 v/v） with the flow rate at 1.00 ml/min. Results：When the silybin was at 1.0 ug/ml - 50 ug/ml, the perfect linearity between concentration and peak area ratio was ob- tained（r = 0. 9998 ）. The detection limit of glutathione was 0.20 ug/ml, the recovery was 94.3% - 98： 0% by adding standard to sample solution and the RSD obtained for the within - run precision was between 2.40% - 2. 66%. Conclusion：This method is simple,rapid and accurate, so it can be used for the determination of silybin in health food.

关 键 词：高效液相色谱法 保健食品 水飞蓟宾 

分 类 号：O657.72[理学—分析化学]