痴呆病人ApoE基因检测与多态性分析  

A study on ApoE in dementia

作　　者：冀成君[1] 金弘敏[1] 

机构地区：[1]北京回龙观医院,北京100096

出　　处：《现代康复》2000年第10期1524-1525,共2页Modern Rehabilitation

摘　　要：目的研究痴呆病人与载脂蛋白Ｅ（ＡｐｏＥ）等位基因之间的关系。方法对３０例老年性痴呆（ＡＤ）患者和２５例多发梗死性痴呆患者的ＡｐｏＥ基因进行检测，确定基因型，计算基因频率，验证ＡｐｏＥ与ＡＤ的相关性。结果ＡＤ病人基因型以ε３／４最多，ε３／３型次之；血管性痴呆（ＶＤ）病人以ε３／３型最多，ε２／３次之；从等位基因频率看，ＡＤ病人及ＶＤ均以ε３型等位基因频率最高，ＡＤ病人ε４等位基因频率显著高于ＶＤ病人及正常人群， ＶＤ各型基因频率与上常人群无差别。结论 ＡｏｐＥ基因频率分布在ＡＤ与 ＶＤ及正常人群之间存在异质性，ε４等位基因是ＡＤ的主要危险因素。Objective To study the relevancy between ApoE gene and the dementia. Method 30 AD patients and 25 vascular dementia patients were detected by polymerase chain reaction and restriction fragment length polymorphism(PCR - RFLP). The products were digested with Hhal and subjected to electrophoresis on polyacrylamids gels. Result The results showed that among ADpatients ApoE gene ε 3/4 is the highest, ε3 /3 is the second, and for vascular dementias ε3/3 is the highest, ε2/3 is the second. The frequency of ε4 is significantly higher in AD than that in control. Conclusion The ApoE gene ε4 is a significant risk factor in AD patients.

关 键 词：痴呆 载脂蛋白E 等位基因 基因频率 

分 类 号：R749.160.3[医药卫生—神经病学与精神病学]