杂交小麦的醇溶蛋白电泳鉴定  

Hybrid Wheat and Their Three Parent Lines Identification by Gliadin Electrophoregrams

作　　者：李玉红[1] 陈鹏[1] 韩战敏 郭蔼光[1] 杨天章 

机构地区：[1]西北农林科技大学,陕西杨陵712100 [2]洛阳农业科学研究所,河南471022

出　　处：《西北农业学报》2000年第3期95-98,共4页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：以 L型杂交小麦及亲本三系为材料 ,比较了 70 %乙醇和 ZY型提取剂提取麦醇溶蛋白的效果 ,并用70 %乙醇做提取剂 ,对 P.Kolster( 1 989)法、改良的 Weghe法和酸性梯度胶分离法进行比较。结果表明 ,改良的 Weghe法和酸性梯度胶分离法效果较好 ,在此基础上 ,对供试的 9个材料进行电泳分析 ,发现用 Weghe法和梯度胶分离法可以鉴别出杂种及常规种之间的明显差异 ,但不能鉴别出 L型、T型不育系及相应保持系之间的明显差异。In this study the gliadin patterns extracted by 70% aqueous ethanol and ZY extraction medium are presented. Three acid electrophoresis methods for the identification of L type hybrid wheat and its parent lines are compared. Modified acid polyacrylamide gel electrophoresis is preferred for materials identification of wheat.It resulted in characteristic protein patterns for all nine wheat materials. L j 1A and L j 1B and F L produced the same pattern.T 0004RdA and T 0004RdB can be distinguished by a very small difference in the region of the slow moving proteins.

关 键 词：小麦杂种 醇溶蛋白 电泳鉴定 品种鉴定 

分 类 号：S512.1[农业科学—作物学]