荧光光谱法研究儿茶素与对磺化杯[n]芳烃的相互作用  

Fluorescence Spectroscopy Studies on the Inclusion Interaction of p-Sulfocalix[n]arenes and Catechins

作　　者：马静[1] 王芳[1] 张国兰[2] 郭文荣[2] 司旭东[2] 卞伟[2] 

机构地区：[1]山西医科大学药学院,太原市新建南路56号030001 [2]山西医科大学基础医学院,太原市新建南路56号030001

出　　处：《光谱实验室》2013年第4期1686-1689,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：山西省高等学校大学生创业基金(2011116;2012103);山西医科大学学生创新基金

摘　　要：采用荧光光谱法在室温下研究了酸性(pH=2.00)、中性(pH=7.00)及碱性(pH=9.00)条件下对磺酸基杯[4]芳烃(SCX4)、对磺酸基杯[6]芳烃(SCX6)及对磺酸基杯[8]芳烃(SCX8)与儿茶素的包合作用。固定儿茶素浓度,逐渐改变对磺化杯[n]芳烃的浓度,儿茶素的荧光强度有规律地降低,表明主-客体包合物的形成。同时用最小二乘法进行非线性拟合计算出包合常数,初步验证了对磺化杯[n]芳烃与儿茶素1:1的包合模式,并且室温条件下,SCX8在pH=9.00的磷酸缓冲体系中对儿茶素的包合能力最强,包合常数为2.769×104L·mol-1。At room temperature,the inclusion interactions of catechins and p-sulfocalix[4]arene（SCX4）,p-sulfocalix[6]arene（SCX6） and p-sulfocalix[8]arene（SCX8） were studied by fluorescence spectroscopy in pH=2.00,pH=7.00 and pH=9.00 conditions.Fixing the concentration of catechins,the fluorescence of catechins was reduced regularly with the addition of p-sulfocalix [n]arenes,which indicated the formation of inclusion compounds.And the association constants of inclusion complexes were calculated by nonlinear fitting method.The result validated preliminarily that the inclusion stoichiometries between catechins and p-sulfocalix[n]arenes are all 1∶1.Furthermore,at room temperature,SCX8 has the strongest inclusion capacity in pH=9.00 phosphate buffer and its association constant is 2.769×104L·mol-1.

关 键 词：儿茶素 对磺酸基杯[n]芳烃 荧光光谱 包合作用 

分 类 号：O657.32[理学—分析化学]