培养条件及培养基组分对粘质沙雷氏菌生长及产D-乳酸的影响  

Effects of Culture Conditions and Medium Components on the Growth and Yielding D-Lactic Acid of Serratia Marcescens

作　　者：满永博[1] 范季瀛[1] 苏刚[1] 饶犇[1] 沈亚领[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237

出　　处：《化学与生物工程》2013年第7期56-60,70,共6页Chemistry & Bioengineering

摘　　要：通过摇瓶实验,确定粘质沙雷氏菌发酵生产D-乳酸的培养条件为:温度34℃,pH值6.5,种子液接种量5%,载液量为150mL/500mL,采用前期通气有氧培养至菌体对数生长后期、而后厌氧发酵产酸的两阶段培养工艺。考察培养基各组分对菌体生长及乳酸产量的影响,确定葡萄糖及酵母粉作为碳、氮源,并补充添加适量的无机氮。培养基各组分如下(g·L-1):葡萄糖15,酵母粉15,KH2PO41.5,K2HPO4.3H2O 2.0,MgSO4.7H2O 1.0,FeSO4.7H2O 0.03,MnSO4.H2O 0.005,以此培养条件及培养基配方进行摇瓶发酵,D-乳酸产量由(13.5±1.29)g.L-1提高至(29.0±1.42)g·L-1,提高一倍以上,光学纯度大于97%。According to flask experiments, the culture conditions of D-lactic acid production by Serratia marcescens are determined as follows： culture temperature of 34℃, pH value of 6.5, the inoculum amount of 5% ,loading liquid of 150 mL/500 mL, two-stage（ pre-aerobic culture to accumulated the thallus,later anaerobic fermentation ） culture method. Effects of medium components on cell growth and lactic acid yield are studied. Glucose is selected as carbon source,yeast is selected as nitrogen source, and meanwhile appropriate metal ions are added into medium. The optimal medium components （g · L^-1） are as follows： glucose 15, yeast 15, KH2 PO4 1.5,K2HPO4· 3H2O 2.0,MgSO4 · 7H2O 1.0,FeSO4 ＊ 7H2O 0.03,MnSO4· H2O 0.005. Under the optimal culture conditions and medium components by shake flask fermentation,D-lactic acid yield increases from （13.5±1.29） g · L^-1 to （29.0±1.42） g · L^-1 with optical purity over 97%.

关 键 词：粘质沙雷氏菌 D-乳酸 培养条件 培养基组分 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]