人β防御素1～4抑菌及盐敏感性研究  

The antibacteria activity and salt sensitivity of human beta defensin 1～4

作　　者：郭峰[1,2] 李涛[2] 韩燃[2] 房华丽[2] 罗森[2] 王琴[2] 高剑峰[1] 王慧[2] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《安徽医科大学学报》2013年第8期885-889,共5页Acta Universitatis Medicinalis Anhui

基　　金：北京市自然科学基金(编号:7122134)

摘　　要：目的对人β防御素(hBD)1～4的抑菌及盐敏感性进行测定并分析其二级结构对抑菌及盐敏感性的影响。方法圆二色谱预测hBD 1～4的二级结构,应用抑菌实验来测定抑菌效力,提高溶液中的NaCl浓度来进行盐敏感性分析。结果获得了4种hBD的圆二色谱谱图并进行分析。实验发现hBD-1抑菌活力低,抑菌谱窄,hBD-2、hBD-4抑菌活力好于hBD-1,抑菌谱也更广,但是对盐耐受力差,而hBD-3在这4种防御素中拥有最强的抑菌活性及耐盐效力。结论 4种hBD具有不同的抑菌活性和盐敏感性。防御素的理化性质与二级结构共同决定了抑菌活力及其盐敏感性。Objective To analyse the effect of their secondary structure on antibacterial activity and salt sensitivity to the human beta defensin 1 - 4. Methods Predict the secondary structure of human beta defensin 1 ~ 4 by circu- lar dichroism spectrum. Measure their biological activities by antibacterial assay, and then add sodium chloride into incubation solution to analyze the salt sensitivity to these defensins. Results The circular dichroism spectrum of these defensins were got and analysed. The antibacterial activity of hBD-1 was weaker than the others, and its anti- bacterial spectrum was very narrow as well. The hBD-2 and hBD-4 had better antibacterial activities than hBD-1, but they were very salt sensitive. The hBD-3 had the strongest antibacterial activity and salt resistant ability among them. Conclusion The four kinds of the human beta defensins have different antibacterial activities and salt sensi- tivity in vitro. Their antibacterial activities and salt sensitivity are determined by their physical and chemical proper- ties and secondary structure.

关 键 词：人β防御素 抑菌活性 盐敏感性 圆二色谱 抗菌谱 

分 类 号：Q93-334[生物学—微生物学] Q71