HPLC-FLD快速测定大豆中的多环芳烃  

Rapid assay of polycyclic aromatic hydrocarbons in soybean by HPLC-FLD

作　　者：俞晔[1,2] 金青哲[1] 王癑 张慧[2] 殷冉[2] 王兴国[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]张家港出入境检验检疫局,江苏张家港215600

出　　处：《中国油脂》2013年第7期72-75,共4页China Oils and Fats

基　　金：国家"十二五"科技支撑计划项目(2012BAK08B03);江苏检验检疫局科技项目(2011KJ44)

摘　　要：建立了大豆中多环芳烃的快速检测方法。采用乙腈-四氢呋喃(1+1)提取大豆中的多环芳烃,并用高效液相色谱-荧光光谱(HPLC-FLD)法测定多环芳烃含量。方法检出限和定量限分别为0.07～0.61μg/kg和0.27～2.04μg/kg,线性范围为2～200μg/kg,回收率在81.7%～96.5%之间,相对标准偏差在2.10%～10.44%之间。A rapid method for detecting polycyclic aromatic hydrocarbons （PAHs） in soybean was devel- oped. After the soybean was treated with acetonitrile - tetrahydrofuran （ 1 ： 1 ） , the PAHs contents were determined by HPLC - FLD. The detection limit and the limit of quantification of the method were 0.07 - 0.61 μg/kg and 0.27 -2.04 μg/kg, respectively. For the twelve PAHs, the method had a good linear range, which was 2 - 200 μg/kg. The recovery rate ranged from 81.7% to 96.5% and the relative standard deviation was in the range of 2.10% - 10.44%.

关 键 词：HPLC—FLD 大豆 多环芳烃 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]