甘油和木糖共发酵生产1,3-丙二醇的研究  

1,3-Propanediol production via cofermentation of glycerol and xylose

作　　者：陈琳[1] 王领民[1] 金平[1] 凌凤香[1] 

机构地区：[1]中国石油化工股份有限公司抚顺石油化工研究院,辽宁抚顺113001

出　　处：《化学工程》2013年第7期5-8,28,共5页Chemical Engineering(China)

摘　　要：以克雷伯氏肺炎杆菌代谢甘油为研究对象,采用木糖作为发酵过程中的辅助底物与甘油共发酵生产1,3-丙二醇,以解决甘油单耗过大的问题,并研究了甘油和木糖共发酵过程中相关代谢物浓度的变化规律。实验表明:克雷伯氏肺炎杆菌可利用D-木糖经过磷酸戊糖途径为菌体代谢提供大量的还原力(NADPH和NADH),促进1,3-丙二醇的合成。与甘油单独发酵相比,以木糖作为辅助底物的微氧批次补料发酵中,1,3-丙二醇的质量浓度、甘油转化率及产量分别提高了10.58%,21.11%和10.98%。共发酵生产1,3-丙二醇在降低甘油到1,3-丙二醇单耗的同时,还可以促进副产物的生成,为克雷伯氏菌发酵多联产工艺提供了可能,从而较全面地降低甘油发酵生产1,3-丙二醇工艺的技术成本,具有一定的研究意义。Taking fermentation of glycerol by Klebsiella pneumoniae as research subject, 1,3-propanediol （PD） was produced by cofermentation of glycerol and xylose （ as eosubstrate ） so as to solve the problem of high glycerol consumption. The changes of relevant metabolites concentration in the cofermentation of glycerol and xylose were also studied. The experimental results show that the synthesis of 1,3-propanediol can be enhanced through the pentose phosphate pathways of D-xylose by Klebsiella pneumoniae, offering more reducing equivalent （ NAPDH and NADH）. Compared with the fermentation of glycerol alone, in fed-batch fermentation with xylose as cosubstrate, the final 1,3-PD mass concentration, conversion rate of glycerol and output are improved by 10.58%, 21.11% and 10.98% , respectively. The glycerol consumption decreases during the cofermentation of glycerol and xylose to produce 1,3-propylene, which can also promote the formation of by-products, allowing a possibility that poly- generation process can be achieved by Klebsiella fermentation, thus reducing comprehensively the cost of 1,3- propanediol production by glycerol fermentation. So it has a certain research significance.

关 键 词：甘油 木糖 克雷伯氏肺炎杆菌 共发酵 1 3-丙二醇 

分 类 号：TQ223.162[化学工程—有机化工]