响应面法优化海带中膳食纤维提取工艺  

Optimization of Extraction Technique of Dietary Fiber from Kelp Laminaria japonica by Response Surface Methodology

作　　者：王明艳[1] 陈丽[1] 张诗山 喻川[1] 沈春阳[1] 

机构地区：[1]淮海工学院江苏省海洋资源开发研究院,江苏连云港222005 [2]连云港天天海藻工业有限公司,江苏连云港222110

出　　处：《水产科学》2013年第6期321-327,共7页Fisheries Science

基　　金：江苏省海洋生物技术重点建设实验室开放课题(2010HS11);连云港市科技攻关项目农业攻关课题(CG1133);淮海工学院大学生创新项目

摘　　要：在单因素试验基础上,以海带膳食纤维得率、膳食纤维膨胀力和持水力(Y1、Y2、Y3)为响应值,利用响应面法对海带膳食纤维提取工艺进行优化。采用响应面法绘制三维及等高线叠加图,应用同步优化程序,得出海带中膳食纤维得率高且生理活性好的最佳提取工艺参数:消化温度为56℃,碱液质量浓度为10.5g/L,消化时间为2h。在此工艺条件下,膳食纤维得率实测值为32.79%,预测值约为32.35%;膨胀力为106.42%,预测值约为106.12%;持水力为1314,预测值约为1318。实测值与预测值非常接近,可见响应面同步优化法对海带膳食纤维提取工艺参数进行优化合理可行。Based on the results of single factor experiments, the extraction conditions of dietary fiber（DF） from kelp Larninaria japonica were optimized by response surface methodology（RSM） with DF extraction yields（Y1）, DF expansive capaeity（Yz） and DF water holding capacity（Y3） as response values. The three dimensions and overlay contour plots revealed that the best extraction of the DF from kelp was observed under the optimum conditions of 56 ℃ digestion temperature, 10. 5 g/L alkali liquor, and 2 h digestion period. Under the optimal conditions, the DF yield, DF expansive capacity and DF water holding capacity （Y1.32. 79%; Y2：106. 42 Y3.1314） were found to be in close agreement with values predicted by the mathematics models, indicating that the RSM is practicable for optimization of digestion procedure, and that will improve the extraction conditions of DF extraction from kelp.

关 键 词：海带 膳食纤维 提取 响应面法 叠加等高线图 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]