高效液相色谱法测定莜麦米酒中γ-氨基丁酸  

Determination of γ-aminobutyric acid in naked oats wine by high performance liquid chromatography

作　　者：刘文勃[1] 易好诚[1] 李苏红[1] 徐杰[1] 王俊伟[1] 张雷[1] 

机构地区：[1]沈阳师范大学粮食学院,辽宁沈阳110034

出　　处：《粮油食品科技》2013年第1期67-70,共4页Science and Technology of Cereals,Oils and Foods

摘　　要：建立紫外—高效液相色谱测定莜麦米酒中的γ-氨基丁酸(γ-Aminobutyric acid,GABA)含量的分析方法。利用碱性条件下,GABA可与邻苯二甲醛(OPA)发生衍生化反应,生成具有较强紫外吸收的衍生物。采用紫外—高效液相色谱仪检测,色谱条件:Eclipse XDB-C18分析柱(5μm,4.6×150 mm),以50 mmol/L的乙酸钠(pH6.8)—甲醇—四氢呋喃(THF,A相,82∶17∶1;B相,22∶77∶1,V∕V)为流动相进行梯度洗脱,可检测到GABA在0.04～0.5 g/L范围内与GABA峰面积的线性关系良好R2=0.9985,样品衍生时间1 min最佳,平均回收率为99.5%,精密度相对标准偏差(RSD)为1.6%,重复性(RSD)为0.66%,最低检出限为0.0254 g/L。方法简单快捷、重复性好、灵敏度高,可用于莜麦、莜麦发酵酒等中GABA含量测定。The content of γ-aminobutyric acid（GABA）in naked oats wine was determined by UV— high performance liquid chromatography.In alkalescence,GABA took derivatization reaction with orthophthalaldehyde（OPA） to create ramification with strong UV—absorptivity,and determined by a UV detector.The separation of GABA was achieved on Eclipse XDB-C18 column（5 μm,4.6×150 mm）using 50 mmol/L of sodium acetate（pH 6.8）-methanol-THF（A,82：17：1;B,22：77：1,V/V） as the mobile phase at a gradient elution.GABA was linear with GABA peak area（R2=0.9985） in the range of 0.04-0.5 g/L,the optimal reaction time was 1 min;The average recovery rate was 99.5%;the relative standard deviation was 1.6% and the repetitiveness 0.66%,and the minimum detecting limit 0.025 4 g/L.The method is simple,rapid with high repetitiveness and sensitivity.It can be widely applied to determine GABA in naked oats and naked oats wine.

关 键 词：莜麦米酒 Γ-氨基丁酸 紫外检测 高效液相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]