生物膜变形链球菌对反义寡核苷酸的摄取率检测  

The rate of Streptococcus mutans uptaking PS-ODN in biofilm

作　　者：李睿[1] 郭青玉[2] 肖刚[2] 邓晓红[3] 

机构地区：[1]宝鸡市口腔医院牙体牙髓病科,721001 [2]西安交通大学口腔医院儿童牙病科 [3]西安交通大学口腔医院牙体牙髓病科

出　　处：《实用口腔医学杂志》2013年第4期487-490,共4页Journal of Practical Stomatology

摘　　要：目的:探讨阳离子聚合物So-fast提高生物膜中变形链球菌对反义寡核苷酸摄取率的效果。方法:制备变形链球国际标准株GS5生物膜,在无载体和用阳离子聚合物作载体So-fastTM情况下用变形链球菌gtfB基因的反义脱氧寡核苷酸PS-ODN转化生物膜中变形链球菌,采用流式细胞仪检测生物膜中变形链球菌细菌对PS-ODN的摄取效率。结果:生物膜中的变形链球菌对裸反义寡核苷酸的摄取效率为16%,采用So-fastTM作为转化载体,摄取效率为71.7%。结论:阳离子聚合物可促进反义寡核苷酸通过变形链球菌细胞膜,使其摄取效率明显提高。Objective：To study the effect of transfection reagent So-fast in the transfection of PS-ODN into S.mutans in biofilm.Methods： Biofilm of S.mutans GS5 was prepared.Transfection of gtfB phosphorothioate antisense oligonucleotide（PS-ODN） into S.mutans GS5 was conducted with or without transfection reagent So-fast^TM.The rate of S.mutans uptaking PS-ODN and PS-ODN/So-fast^TM compound was measured by flow cytometry.Results： The rate of S.mutans uptaking PS-ODN in biofilm was 16%.The rate of uptaking PS-ODN/So-fast^TM compound in biofilm was 71.4%.Conclusion： Cationic polymer So-fast may increase the uptaking rate of PS-ODN into S.mutans.

关 键 词：变形链球菌 龋病 生物膜 反义寡核苷酸 

分 类 号：R780.2[医药卫生—口腔医学]