表面解吸常压化学电离质谱快速鉴别硫磺熏蒸八角  

Rapid Screening of Sulfur Fumigated Chinese Star Anises by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

作　　者：罗丽萍[1] 王姜[2] 章文军[3] 戴喜末[1,2] 方小伟[2] 张茜[1,2] 刘亚丽[2,3] 陈焕文[2] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330047 [2]东华理工大学江西省质谱科学与仪器重点实验室,南昌330013 [3]河北工业大学化工学院,天津300130

出　　处：《分析化学》2013年第7期1050-1056,共7页Chinese Journal of Analytical Chemistry

基　　金："十二五"农村领域国家科技计划基金(No.2012BDA29B01-3);国产科学仪器自主创新能力培育与应用示范基金(No.PXM2011_178305_113597);江西省科技厅基金(No.2010BNB00900)资助项目

摘　　要：采用自行研制的表面解吸常压化学电离质谱(DAPCI-MS),无需样品预处理,对硫磺熏蒸八角和未熏八角直接进行正、负离子模式检测,获得其化学指纹图谱,并通过主成分分析(PCA)及聚类分析(CA)方法对所获指纹谱图信息进行分析,进而对不同样品进行鉴别。结果表明,在正、负离子模式下,DAPCI-MS都可对八角表面多种特征化学成分进行分析,快速获得八角的化学指纹谱图,并能够对目标组分进行多级串联质谱鉴定,结合PCA及CA方法可对八角是否经硫磺熏蒸进行快速鉴别。本方法无需样品预处理,灵敏度高,分析速度快,无污染,可望应用于市场上硫熏制品的快速鉴别。Without any sample pretreatment, the mass spectral fingerprints of sulfur fumigated Chinese star anises and untreated samples were rapidly obtained in either a positive or negative ion detection mode with a home-made surface desorption atmospheric pressure chemical ionization （DAPCI） source. The DAPCI-MS raw data were further analyzed by principal component analysis （PCA） and cluster analysis （CA） , and several unknown samples were successfully discriminated using the PCA model. The results showed that the DAPCI- MS was able to detect some characteristic chemicals from the Chinese star anises＇ surface and identify the components by tandem mass spectrometry （MS/MS）. The further principal component analysis （PCA） and cluster analysis （CA） of MS fingerprints allow a confident discrimination of sulfur fumigated star anise samples from the non-sulfur fumigated samples. The method developed here is attractive to provide a fast and effective way to screen sulfur fumigated products with sufficient sensitivity and no toxin pollution. This method is expec- ted to be applied to rapid identification of the sulfur fumigated product on the market in future.

关 键 词：表面解吸常压化学电离 质谱 主成分分析 聚类分析 硫磺熏蒸 八角 

分 类 号：TS264[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]