柑橘大实蝇微卫星标记的筛选  

Screening of Microsatellite Markers in Bactrocera minax (Diptera: Tephritidae)

作　　者：高立志[1] 刘映红[1] 万宣伍[1,2] 王佳[1] 洪枫[1] 

机构地区：[1]西南大学昆虫学研究所,重庆400716 [2]四川省植物保护站,成都610041

出　　处：《中国农业科学》2013年第15期3285-3292,共8页Scientia Agricultura Sinica

基　　金："973"计划前期研究专项(2009CB125903)

摘　　要：【目的】筛选可用于柑橘大实蝇(Bactrocera minax)种群遗传研究的微卫星标记。【方法】选取33对柑橘大实蝇同属种柑橘小实蝇(B.dorsalis)、木瓜实蝇(B.papayae)、瓜实蝇(B.cucurbita)、东澳番茄实蝇(B.cacuminata)中多态性较好微卫星引物,通过对PCR扩增产物进行琼脂糖电泳初筛选及变性聚丙烯酰胺凝胶电泳再筛选,并对其等位基因频率、多态信息含量和有效等位基因数等指数进行分析。【结果】33对微卫星引物中有12对引物对柑橘大实蝇有扩增条带,其同源率为36.4%。6个微卫星位点在柑橘大实蝇上共检测到23个等位基因,平均等位基因数为3.83个;6对微卫星引物的平均多态信息含量(PIC)为0.2248,最高为0.4105,最低为0.0869。【结论】所筛选出的6对微卫星引物表现出较好的多态性,可用于后续对柑橘大实蝇的种群遗传研究。【Objective】The objective of the study is to screen some highly polymorphic microsatellite markers for research on the population genetic structure of Chinese citrus fly（Bactrocera minax）.【Method】Thirty-three pairs of microsatellite primers were selected from B.dorsalis,B.papayae,B.cucurbitae and B.cacuminata,which belonged to the same genus with B.minax.Then these primers were firstly screened through agarose gel electrophoresis of the PCR amplification product and rescreened through modified polyacrylamide gel electrophoresis.At last,the allele frequency,polymorphism information content,effective number of alleles and so on were used for analysis.【Result】There were only 12 pairs of microsatellite primers amid 33 pairs that could amplify the genomes of B.minax specifically,and the homologous rate was 36.4%.Twenty-three alleles were detected at the six microsatellite sites in B.minax,and the average number of alleles was 3.83.The average polymorphism information content of the six microsatellite primers was 0.2248,the highest was 0.4105,and the lowest was 0.0869.【Conclusion】The screened 6 microsatellite primers of B.minax showed a good polymorphism,and could be used in the subsequent research.

关 键 词：柑橘大实蝇 微卫星引物 等位基因 多态性 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]