葡萄籽原花青素对Ⅱ型糖尿病大鼠皮肤胶原代谢的影响  

Effect of Grape Seed Proanthocyanidins Extract (GSPE) on Skin Collagen Metabolism in Type Ⅱ Diabetic Rats

作　　者：蔡夏夏[1] 李雪[1] 鲍雷[1] 丁叶[1] 张召锋[1] 李宇杰[1] 井路路[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191

出　　处：《食品科学》2013年第15期213-218,共6页Food Science

基　　金：国家自然科学基金项目(81072293;81102116)

摘　　要：目的:观察葡萄籽原花青素(GSPE)对Ⅱ型糖尿病大鼠胶原代谢的影响,并进一步探讨可能的作用机制。方法:雄性SD大鼠高脂饲料喂养4周后,腹腔注射0.01mol/L链脲佐菌素(STZ,剂量30mg/kg)建立Ⅱ型糖尿病大鼠模型,成模后随机分为糖尿病(DM)对照组及原花青素低、中、高剂量干预组;基础饲料饲养的大鼠随机分为正常对照组和原花青素对照组,原花青素对照组的剂量为250mg/(kg.d)。原花青素低、中、高剂量干预组的剂量分别为125、250、500mg/(kg.d)。连续饲养12周,测定体质量,空腹血糖值、皮肤糖浓度及羟脯氨酸含量;光学显微镜下观察皮肤结构及胶原纤维变化,免疫组化法观察脚垫胰岛素样生长因子-1(IGF-1)和Ⅲ型胶原(CollagenⅢ)的表达。结果:GSPE干预组与DM对照组相比空腹血糖值均有所下降,但无显著性差异(P>0.05);GSPE高剂量组可以明显降低皮肤组织糖含量(P<0.001),GSPE干预组均能明显增加皮肤羟脯氨酸含量(P<0.05);组织学上,GSPE能够抑制糖尿病大鼠的皮肤变薄,GSPE高剂量干预组能够增加脚垫IGF-1和Ⅲ型胶原的表达。结论:葡萄籽原花青素对Ⅱ型糖尿病大鼠胶原代谢有一定改善作用。Purpose： To observe the effect of grape seed proanthocyanidins extract （GSPE） on skin collagen metabolism and its possible mechanism in type II diabetic rats. Methods： In order to establish type II diabetic rat model, male SD rats were injected with streptozotocin （STZ, 0.01 mol/L, 30 mg/kg） after being fed high-fat diet for 4 weeks. Then the diabetic rats were randomly divided into diabetes-vehicle group （DM control group） and three GSPE treatment groups. Rats fed normal diet were randomly divided into normal and GSPE control group. The dose of GSPE control group was 250 mg/（kg,d）, and the doses of three GSPE treatment groups were 125, 250, 500 mg/（kg d）, respectively. After administration for 12 weeks, the body weight, fasting blood glucose （FBG）, skin glucose concentration and hydroxyproline content were measured. Paraffin-embedded skin sections were stained by hematoxylin-eosin （HE） and Masson, and then examined under a microscope. The thickness of skin was measured with Image-Pro Plus 6.0. Insulin like growth factor-1 （IGF-1） and collagen III in rat footpad were detected by immunohistochemical method. Results： Compared with the DM control group, FBG values were decreased but there was no significant difference in three GSPE treatment groups （P 〉 0.05）. GSPE at the high dose revealed a significant reduction in glucose concentration of skin tissues when compared with the DM control group （P 〈 0.001）. In contrast, each GSPE dose group could significantly increase skin hydroxyproline content （P 〈 0.05）. After staining skin tissues by HE and Masson, we observed that GSPE could inhibit the skin to be thin in diabetic rats. Compared with the DM control group, the footpad in the GSPE high-dose group had higher IGF-1 and collagen III expression. Conclusion： GSPE has improvement effect on skin collagen metabolism of type II diabetic rats.

关 键 词：葡萄籽原花青素 Ⅱ型糖尿病 胶原代谢 胰岛素样生长因子-1 Ⅲ型胶原 

分 类 号：R151.2[医药卫生—营养与食品卫生学]