大鼠急性肠缺血模型中血清肠脂肪酸结合蛋白和D-乳酸浓度与缺血时间和肠组织损伤的相关性  

Correlation of serum intestinal fatty acid binding protein and D-lactate with ischemic time and intestinal injury in acute mesenteric ischemia rats

作　　者：石卉[1] 吴本俨[1] 王昌正[1] 王卫华[1] 刘文徽[1] 

机构地区：[1]解放军总医院南楼临床部消化内科,北京100853

出　　处：《中华老年多器官疾病杂志》2013年第7期526-529,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：中央保健专项资金科研课题（B2009A105）;解放军总医院南楼创新基金

摘　　要：目的 探索大鼠急性肠缺血模型的血清肠脂肪酸结合蛋白（I-FABP）和D-乳酸（D-Lac）浓度与缺血时间、肠黏膜损伤的相关性。方法 建立大鼠肠系膜前动脉缺血模型，按照缺血时间随机分为6组：假手术组（空白对照），缺血30min，60min，90min，120min，150min组，每组10只。ELISA法分析各组血清I-FABP和D-Lac水平。免疫组化法评估受损肠黏膜组织I-FABP含量。对病变组织进行损伤评分，评估血清I-FABP和D-Lac水平与组织损伤的相关性。结果 大鼠肠缺血30min时血清I-FABP浓度明显升高，于90min达到峰值[（1741.37±184.12）mg/L]，I-FABP抗体染色阳性率达 72.5% （P＜0.05）；大鼠肠缺血60min时血清D-Lac浓度开始升高，随缺血时间延长，D-Lac浓度升高越明显（P＜0.05）。小肠黏膜损伤评分结果提示，缺血时间越长，组织损伤评分越高。Spearman等级相关非参数检验结果表明，血清D-Lac浓度与组织损伤正相关（r＝1）；血清I-FABP浓度与组织损伤评分无相关性（r＝0.6）。结论 血清I-FABP在大鼠急性肠缺血早期明显升高，达到峰值后随缺血时间的延长而下降，血清D-Lac在缺血中后期升高明显，可反映肠组织损伤程度。Objective To investigate the correlation of the serum levels of intestinal fatty acid binding protein （I-FABP） and D-lactate （D-Lac） with ischemic time and intestinal mucosa injury in rats with acute mesenteric ischemia. Methods A total of 60 rats were randomly and equally divided into 6 groups, that is, sham-operation group and 5 mesenteric ischemia groups according to mesenteric artery occluded for 30, 60, 90, 120 and 150 min respectively. Their serum levels of I-FABP and D-Lac were determined by ELISA. The content of I-FABP in the damaged intestinal mucosa was measured by immunohistochemical assay. Histopathological analysis was performed in the injured intestinal mucosa to evaluate the score of injury. The correlation of serum levels of I-FABP and D-Lac with tissue injury was analyzed. Results The serum I-FABP level was significantly increased from 30 min after ischemia, and reached a peak at 90 min [（1741.37±184.12）mg/L], and the cells positive to I-FABP accounted for 72.5% （P〈0.05）. The serum level of D-Lac was significantly increased from 60 min after ischemia and showed a time-dependent manner （P〈0.05）. The score of injury was also increased with the extent of ischemic time. Spearman’s rank correlation non-parameter test indicated that the serum level of D-Lac was positively correlated with intestinal mucosa injury （r=1）, but that of I-FABP had no such correlation （r=0.6）. Conclusions Serum I-FABP is increased at the early stage of acute mesenteric ischemia, and then after reaching peak, decreased with the elapse of ischemic time. Serum D-Lac is obviously increased at the late stage, and might be used to predict the severity of intestinal ischemic injury.

关 键 词：脂肪酸结合蛋白质类 乳酸 缺血 

分 类 号：R574[医药卫生—消化系统]