红茶浸出物抑制人舌鳞状细胞癌细胞增殖活力的实验研究  

The basic study of the proliferation inhibition effect of black tea extraction in oral cavity cancer cells

作　　者：张晨 王子硕[3] 吴荣琳 李国超 洪虓[1] 黄珊姗[1] 马腾飞[1] 王元银[1] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医学高等专科学校口腔系,合肥230601 [3]合肥市第五十中学,九七班230032 [4]南昌佳美美容医院美容牙科,江西330001

出　　处：《安徽医学》2013年第7期861-864,共4页Anhui Medical Journal

基　　金：国家自然科学基金项目(编号:81271162);安徽省自然科学基金项目(编号:11040606M204);安徽省教育厅重点项目(编号:KJ2012A172)

摘　　要：目的本文研究红茶浸出物抑制体外培养人舌鳞状细胞癌Tca8113细胞增殖效应及其对细胞内硫氧还蛋白还原酶(TrxRs)活力的影响。方法 MTT法检测红茶浸出物及不同程度EGCG氧化产物体外抑制癌细胞增殖效应,金抑制法测定细胞内TrxRs活力水平。结果结果表明,本研究制备的EGCG各种氧化产物在浓度100μmol时均具有与其单体相当的抑癌活性;红茶浸出物抑制增殖表现剂量和时间效应,IC50剂量为250μg/ml。经红茶浸出物处理的细胞内TrxRs活力显著降低(P<0.01)。结论红茶浸出物抑制人舌鳞状细胞癌Tca8113细胞体外增殖,其机制涉及打击细胞内TrxRs活力。Objective The present study investigated the proliferation inhibition effect of black tea extraction in oral cavity cancer cells and the potential mechanism related of the impact on intracellular thioredoxin reductase activity. Methods MTT assay was performed to determine the proliferation inhibition effect of black tea extraction and oxidized products of EGCG in vitro, and intracellular thioredoxin re- ductase activity was determined by auranofin inhibition assay. Results The results showed all oxidized products of EGCG exhibited equiva- lent inhibition ability with EGCG at concentration of 100 μmol. Dose and time response were certified of inhibition effect of the black tea ex- traction with ICS0 = 250μg/ml. Intracellular thioredoxin reductase activity was significantly reduced upon the treatment of black tea extrac- tion （ P 〈 0.01 ）. Conclusion Black tea extract inhibits Tca8113 proliferation via reducing TrxR activity.

关 键 词：红茶 人舌鳞状细胞癌Tca8113细胞 硫氧还蛋白还原酶 

分 类 号：R739.8[医药卫生—肿瘤]