大亚湾海域可培养细菌鉴定及系统发育分析  

Identification and phylogenetic analysis of culturable bacteria in Daya Bay

作　　者：江晓亮[1] 王朝晖[1] 王剑[1] 熊毅俊 林朗聪[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632

出　　处：《生态科学》2013年第3期290-297,共8页Ecological Science

基　　金：国家自然科学基金项目(41276154;41076093)

摘　　要：对大亚湾海域表层水体的可培养细菌进行分离,测定了分离培养细菌的16S rRNA基因V3区序列,并采用该研究中的35个序列与从GenBank下载的46个序列构建了系统树,对细菌进行了分子鉴定。在9个站点的表层水体中共分离得到70株菌株,分属35个种。其中α-变形菌纲(Alphaproteobacteria)和放线菌纲(Actinobacteria)为分离菌株最多的两个菌纲,分离率分别为40%和35.7%;而β-变形菌纲(Betaproteobacteria)仅分离到1株(1.4%)。此外,还分离得到盖球菌属(Kytococcus)、间胞囊菌属(Intrasporangium)等非常见菌株。大亚湾海域细菌的Shannon-Wiener`s多样性指数为3.21。大亚湾海域表层水体可培养细菌丰富的种类多样性以及较高的α-变形菌分离率,说明了该海域具有良好的水质,同时也是潜在的微生物菌种资源库。Culturable bacter/a were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and lntrasporangium, which were rarely reported, were also isolated. The value of Shaunon-Wiener＇s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.

关 键 词：大亚湾 可培养细菌 16SRRNA基因 系统发育分析 

分 类 号：Q939[生物学—微生物学]