一个新的生长激素受体相关蛋白的发现及临床意义  

A New Discovery of Growth Hormone Receptor,Related Protein and Its Clinical Significance

作　　者：陈千[1] 刘莹[2] 杨帆[2] 晏慧超 万瑜[2] 

机构地区：[1]中山大学新华学院医学系,广东广州510520 [2]武汉大学基础医学院生理教研室 [3]武汉大学海外开放实验室

出　　处：《华南国防医学杂志》2013年第7期468-470,共3页Military Medical Journal of South China

基　　金：教育部高等学校博士学科点专项科研基金(20120171120116)

摘　　要：目的鉴定实验中发现的一个新蛋白与生长激素受体(growth hormone receptor,GHR)蛋白的相关性。方法用两种不同的GHR抗体,通过蛋白质印迹法(Western blot)检测16对结直肠癌患者的肿瘤组织和癌旁组织以及8个结直肠癌细胞系中GHR的表达;在结直肠癌细胞系中过表达pcDNA3.1-HA-GHR质粒,并应用HA-tag血凝素标记(hemagglutinin-tag,HA-tag)抗体检测HA融合蛋白。结果用两种不同的GHR抗体在结直肠癌患者组织中及结直肠癌细胞系中均能检测到一大小约45kD的蛋白,抗HA-tag抗体检测外源性GHR的表达也能检测到一与HA融合的大小约45kD的蛋白。并且发现这个45kD大小的蛋白在癌组织的表达明显低于癌旁组织(P<0.05),在8种结直肠癌细胞系均检测到这一蛋白,其大小为45kD,并通过HA-tag抗体检测到此蛋白与GHR蛋白有关。结论此蛋白与GHR有关,并且可能参与了结直肠癌的发生与发展。Objective To identify a novel growth hormone receptor （GHR） related protein and its clinical signifi- cance. Methods Western blot was employed to test the expression of GHR at 16 paired colorectal cancer with their adja- cent mucosa and 8 colorectal cancer cell lines. Exogenous GHR was over-expressed in colon cancer cell line through HA tagged GHR expression plasmid. HA fusion protein with anti-HA-tag primary antibody was tested. Results A new 45kD protein was tested by two different anti-GHR antibodies in colorectal cancer tissues and cell lines. A novel HA-tagged GHR related protein about 45kD can also be tested by anti-HA tag antibody. The expression of the new 45kD GHR relat- ed protein was decreased significantly in colorectal cancer tissue compared with adjacent mucosa. Conclusion A novel GHR related protein with about 45kD is found. Down-regulation of the novel GHR related protein may involve in the car- cinogenesis and development of colorectal cancer.

关 键 词：生长激素受体 结直肠癌 相关蛋白 

分 类 号：R735.3[医药卫生—肿瘤]