固相萃取-高效液相色谱法同时测定食品中七种合成色素  

Simultaneous determination of seven kinds of synthetic pigments in foods by SPE-HPLC

作　　者：文君[1] 孙丹红[1] 胡晓科[1] 

机构地区：[1]成都市疾病预防控制中心,成都610041

出　　处：《中国卫生检验杂志》2013年第7期1717-1719,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:采用固相萃取-高效液相色谱法同时测定食品中七种合成色素(柠檬黄、苋菜红、靛蓝、胭脂红、日落黄、诱惑红、亮蓝)。方法:样品经水溶解后,选用StrataX-AW SPE弱阴离子小柱净化,HPLC测定。色谱条件:Agilent C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.02 mol/L磷酸二氢钾水溶液(用NaOH调至pH5.8),流速1.0 ml/min,进样量10μl,柱温35℃。检测波长:428 nm,520 nm,620 nm,梯度洗脱。结果:七种色素在0.20 mg/L^5.00 mg/L范围内与响应值呈线性关系,不同水平加标回收率为85%~110%,RSD为2.1%~4.8%,最低检出浓度均小于或等于0.001g/kg。结论:方法简便、快速、灵敏、准确,适用于食品中七种色素的同时测定。Objective： To develop a method for the simultaneous determination of seven kinds of synthetic pigments in foods by SPE - HPLC. Methods： The homogenized samples were dissolved by water and purified by StrataX - AW SPE column. HPLC was applied for quantitative detection of the pigments in the samples. The target samples were separated on an Agilent - C18 column（4.6 mm × 250 mm, 5 μm） with the mobile phase of methanol - KH2PO4 for gradient elution and detected using a DA detector at 428nm, 520nm, 620nm. The injection volume is 10μl. The column temperature is 35 ℃. The flow rate was 1 ml/min. Results： This method had good linearity between 0.20 mg/L- 5.00 mg/L and good precision （RSD 〈 5% ）. The average rates of recovery were 85% - 110%. The detection limit were lower than 0.001 g/kg. Condusion： The method is simple, sensitive, accurate and renrodueihle.

关 键 词：高效液相色谱法 食品 合成色素 

分 类 号：O657.72[理学—分析化学]