流加葡萄糖策略对盐霉素生物合成调控影响  

Effect of glucose feeding strategy on the salinomycin biosynthesis in Streptomyces albus

作　　者：高圣君[1] 杭海峰[1] 陈中兵 郭美锦[1] 储炬[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]浙江升华拜克生物股份有限公司,德清313220

出　　处：《中国抗生素杂志》2013年第8期579-583,共5页Chinese Journal of Antibiotics

基　　金：国家高技术研究计划("863"计划)项目<生物过程关键技术及装备研发>(2012AA021201);南太湖特聘专家计划

摘　　要：运用具有多参数检测的生物反应器以及借助BIOSTAR数据处理软件包,在盐霉素原发酵工艺多参数相关性分析的基础上,研究不同阶段流加葡萄糖对盐霉素发酵的影响。结果发现:盐霉素发酵可分为分批发酵期、糖油代谢转化期和糖油转换后期三个阶段,并在糖油代谢转换期中,pH会逐步升高形成一个波峰,而OUR大幅度下降形成一个波谷,这是盐霉素发酵过程中的重要特征;在盐霉素糖油转化阶段流加葡萄糖,菌体代谢出现混沌现象,不利于盐霉素的生物合成;而在糖油转化后并当OUR处于稳定期时流加葡萄糖,此时有利于盐霉素的生物合成,比原发酵工艺效价提高了13.2%。A 50L bioreactor with multi-parameter monitoring and BIOSTAR data processing software package were employed to analyze the metabolic characteristics of salinomycin in Streptomyces albus. On the basis of analyzing the original fermentation process of salinomycin, the effects of glucose feeding strategy at different fermentation phases on the salinomycin biosynthesis were investigated in the present work. The result showed that the fermentation process of salinomycin could be divided into three phases which were batch fermentation phase, transition phase from sugar to oil and the phase after transition. In the transition phase, the curve of pH and OUR formed crest and trough separately, which was the important characteristic of salinomycin fermentation. Addition of glucose at transition phase from sugar to oil led to the secondary metabolism disorder of S. albus, which was detrimental to the biosynthesis of salinomycin. On the other hand, it benefited the salinomycin production when feeding glucose at the stable phase of OUR after transition phase and its titer was 13.2% higher than that of original fermentation process.

关 键 词：盐霉素 生物合成 流加策略 参数相关性分析 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]