蜜蜂肽Apidaecin突变体的体外稳定性  

Stability study of Apidaecin 1Band its mutants in vitro

作　　者：胡胜兰[1] 李卫芬[1] 孙建栋[1] 林志伟[1] 曹翠平[1] 周绪霞[2] 

机构地区：[1]浙江大学动物科学学院教育部动物分子营养重点实验室,浙江杭州310058 [2]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《中国兽医学报》2013年第8期1259-1262,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30901044);国家"十二五"科技支撑计划资助项目(NC2010CA0026)

摘　　要：利用化学合成的方法获得蜜蜂肽Apidaecin 1B(AP 1B)及其系列突变体,通过对不同细菌的MIC测定,筛选获得了抑菌活性更高的改良肽AP2。AP2的特性研究结果表明,在低pH值条件下对胃蛋白酶稳定性好,并且对热、模拟肠液有一定的耐受性。研究结果将为蜜蜂肽Apidaecin的开发及其在畜牧业中的应用奠定基础。Antibacterial peptide Apidaecin 1B and 6 of its mutants were prepared by chemical syn-thesis method. One of the mutants, AP2, with higher antibacterial activity was obtained through minimum inhibitory concentration （MIC） value determination assay. The characteristic of AP2 re-search showed that it had good stability to pepsin in the low pH condition,and could tolerate the heat and the artificial intestinal fluid in some degree. This discovery would provide a theoretical ba- sis for the development of Apidaeein and its application in animal husbandry.

关 键 词：蜜蜂肽 抗菌活性 热稳定性 模拟胃液 模拟肠液 

分 类 号：S859.79[农业科学—临床兽医学]