千佛菌抗衰老作用实验研究  

Study on anti-aging mechanism of Grifola frondosa

作　　者：胥冰[1] 席孝贤[1] 贺新怀 

机构地区：[1]陕西中医学院,陕西咸阳712046

出　　处：《食品工业科技》2013年第16期351-352,358,共3页Science and Technology of Food Industry

基　　金：陕西省科技厅自然科学基础研究项目(2007C228)

摘　　要：目的:观察千佛菌对衰老小鼠血清、脑、肝脏组织抗氧化系统的影响,探讨千佛菌抗衰老的作用机理。方法:采用腹腔注射D-半乳糖制备衰老小鼠模型,并以千佛菌100%浓度(大剂量)、50%(小剂量)浓度给小鼠灌胃治疗,六周后,处死动物。检测血清、脑、肝组织中SOD、GSH-Px、CAT的活性及MDA的含量。结果:千佛菌能显著提高血清、脑及肝组织中SOD、GSH-Px、CAT(p<0.05)活性,降低血清、肝脏和脑组织中MDA(p<0.01)含量。结论:千佛菌通过提高抗氧化酶活性,显著提高衰老小鼠的抗氧化能力,以大剂量疗效显著。Objective：To investigate the protective effect and anti -oxidative activity mechanism of Grifola frondosa in serum,heart and liver of aged mice. Methods：The aging mice were established with D-galactose by intraperitoneal injection. Grifola frondosa was administrated to mice by gavage 100%（Grifola frondosa large dose）,50% （Grifola frondosa small dose. After six weeks,the mice were executed. The serum,heart and liver tissue of mice were collected. The content of malondialdehyde（MDA）,activity of superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） catalase（CAT） were examined by the ultraviolet spectrophotometer detection. Results：Compared with the D-galactose model group,Grifola frondosa in different doses significantly improved the SOD,CAT,GSH-Px activities（p＆lt;0.05） and reduced the MDAcontent（p＆lt;0.01） in serum,heart and brain of mice. Conclusion：Grifola frondosa possesses anti-oxidant activity to protect body against free radical-induced damage,especially for the high dose.

关 键 词：千佛菌 D-半乳糖 衰老 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]