56例IgD型多发性骨髓瘤的分型研究  

Study on 56 Cases of IgD Multiple Myeloma Typing

作　　者：颜绵生[1] 梁鉴波[1] 王松子[1] 廖慧芳[1] 

机构地区：[1]中山大学附属第一医院检验医学部,广东广州510080

出　　处：《中国医药指南》2013年第20期433-434,共2页Guide of China Medicine

摘　　要：目的探讨免疫固定电泳技术在IgD型多发性骨髓瘤(MM)分型中的应用价值,研究其实验室特征。方法从1356例MM患者中,筛选出在免疫固定电泳结果中只有轻链而不出现IgGAM重链的血清样本,用抗游离轻链的单克隆抗体及抗IgD、抗IgE单克隆抗体再进行免疫固定电泳,对IgD型M蛋白阳性患者血清进行免疫球蛋白定量,对其尿液标本进行本周蛋白定性检测。结果 1356例MM患者中有56例为IgD型,占MM的4.1%,其中53例为IgD-λ型,3例为IgD-κ型;51例呈血清游离轻链阳性;56例患者中仅2例尿液本周氏蛋白呈阴性,54例呈阳性;患者血清的IgG、IgA、IgM与正常人比较结果偏低。结论利用抗游离轻链的单克隆抗体及抗IgD、抗IgE抗体进行免疫固定电泳,对多发性骨髓瘤的分型诊断具有重要意义。Objective To investigate the applicability and laboratory attributes of IgD multiple myeloma（MM） typing in the immunofixation electrophoresis. Methods Samples contained the light chains without IgGAM heavy chain were selected from 1356 MM patients. Both the anti-free- light-chain monoclonal antibody and anti-IgD and anti-IgE of monoclonal antibody were used to detect IgD M-protein-positive serum for immunoglobulin quantitative analysis. As the qualitative detection of their urine samples, a Bence-Jones protein test was to apply. Results 56 cases were detected with the IgD type which was 4.1%, of the total 1356 MM patients. 53 and 3 cases were IgD-~.-type and IgD-g respectively while 51 cases were reported serum-free- light chain-positive. Only two cases of the urine Bence-Jones protein were negative, whereas predominance was positive. Lower common value of IgG, IgA, IgM were discovered in patients＇ serum. Conclusion The combination use of anti-free-light-chain monoclonal antibody and anti-IgD and anti-IgE antibody alike for genotyping of multiple myeloma＇s diagnosis has a significant impact on immunofixation electrophoresis. The per se status may be a good predictive marker of sensitivity.

关 键 词：多发性骨髓瘤 IGD 抗体 单克隆 

分 类 号：R733.3[医药卫生—肿瘤]